Determination of Genetic Diversity Using 15 Simple Sequence Repeats Markers in Long Term Selected Japanese Quail Lines

Japanese quail is still used as a model for poultry research because of their usefulness as laying, meat, and laboratory animals. Microsatellite markers are the most widely used molecular markers, due to their relative ease of scoring and high levels of polymorphism. The objective of the research wa...

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Publié dans:Asian-Australasian journal of animal sciences. - 1998. - 29(2016), 12 vom: 20. Dez., Seite 1696-1701
Auteur principal: Karabağ, Kemal (Auteur)
Autres auteurs: Balcıoğlu, Murat Soner, Karlı, Taki, Alkan, Sezai
Format: Article
Langue:English
Publié: 2016
Accès à la collection:Asian-Australasian journal of animal sciences
Sujets:Journal Article Breeding Genetic Diversity Quail Selection Simple Sequence Repeats
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520 |a Japanese quail is still used as a model for poultry research because of their usefulness as laying, meat, and laboratory animals. Microsatellite markers are the most widely used molecular markers, due to their relative ease of scoring and high levels of polymorphism. The objective of the research was to determine genetic diversity and population genetic structures of selected Japanese quail lines (high body weight 1 [HBW1], HBW2, low body weight [LBW], and layer [L]) throughout 15th generations and an unselected control (C). A total of 69 individuals from five quail lines were genotyped by fifteen microsatellite markers. When analyzed profiles of the markers the observed (Ho) and expected (He) heterozygosity ranged from 0.04 (GUJ0027) to 0.64 (GUJ0087) and 0.21 (GUJ0027) to 0.84 (GUJ0037), respectively. Also, Ho and He were separated from 0.30 (L and LBW) to 0.33 (C and HBW2) and from 0.52 (HBW2) to 0.58 (L and LBW), respectively. The mean polymorphic information content (PIC) ranged from 0.46 (HBW2) to 0.52 (L). Approximately half of the markers were informative (PIC≥0.50). Genetic distances were calculated from 0.09 (HBW1 and HBW2) to 0.33 (C and L). Phylogenetic dendrogram showed that the quail lines were clearly defined by the microsatellite markers used here. Bayesian model-based clustering supported the results from the phylogenetic tree. These results reflect that the set of studied markers can be used effectively to capture the magnitude of genetic variability in selected Japanese quail lines. Also, to identify markers and alleles which are specific to the divergence lines, further generations of selection are required 
650 4 |a Journal Article 
650 4 |a Breeding 
650 4 |a Genetic Diversity 
650 4 |a Quail 
650 4 |a Selection 
650 4 |a Simple Sequence Repeats 
700 1 |a Balcıoğlu, Murat Soner  |e verfasserin  |4 aut 
700 1 |a Karlı, Taki  |e verfasserin  |4 aut 
700 1 |a Alkan, Sezai  |e verfasserin  |4 aut 
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