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231224s2016 xx |||||o 00| ||eng c |
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|a 10.5713/ajas.16.0051
|2 doi
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|a pubmed25n0867.xml
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|e rakwb
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|a eng
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| 100 |
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|a Cho, Jin Hyoung
|e verfasserin
|4 aut
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| 245 |
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|a Regional Differences of Proteins Expressing in Adipose Depots Isolated from Cows, Steers and Bulls as Identified by a Proteomic Approach
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 29.06.2016
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Adipose tissue in the loin muscle area of beef cattle as a marbling factor is directly associated with beef quality. To elucidate whether properties of proteins involved in depot specific adipose tissue were sex-dependent, we analyzed protein expression of intramuscular adipose tissue (IMAT) and omental adipose tissue (OMAT) from Hanwoo cows, steers, and bulls of Korean native beef cattle by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic analysis, quantitative polymerase chain reaction (PCR) and western blot analysis. Two different adipose depots (i.e. intramuscular and omental) were collected from cows (n = 7), steers (n = 7), or bulls (n = 7). LC-MS/MS revealed a total of 55 and 35 proteins in IMAT and OMAT, respectively. Of the 55 proteins identified, 44, 40, and 42 proteins were confirmed to be differentially expressed in IMAT of cows, steers, and bulls, respectively. In OMAT of cows, steers, and bulls, 33, 33, and 22 were confirmed to be differentially expressed, respectively. Tropomyosin (TPM) 1, TPM 2, and TPM3 were subjected to verification by quantitative PCR and western blot analysis in IMAT and OMAT of Hanwoo cows, steers, and bulls as key factors closely associated with muscle development. Both mRNA levels and protein levels of TPM1, TPM2, and TPM3 in IMAT were lower in bulls compared to in cows or steers suggesting that they were positively correlated with marbling score and quality grade. Our results may aid the regulation of marbling development and improvement of meat quality grades in beef cattle
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|a Journal Article
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|a Adipose Depots
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|a Hanwoo
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|a Liquid Chromatography-tandem Mass Spectrometry
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|a Marbling
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|a Meat Quality
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| 700 |
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|a Jeong, Jin Young
|e verfasserin
|4 aut
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|a Lee, Ra Ham
|e verfasserin
|4 aut
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|a Park, Mi Na
|e verfasserin
|4 aut
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|a Kim, Seok-Ho
|e verfasserin
|4 aut
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|a Park, Seon-Min
|e verfasserin
|4 aut
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|a Shin, Jae-Cheon
|e verfasserin
|4 aut
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|a Jeon, Young-Joo
|e verfasserin
|4 aut
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|a Shim, Jung-Hyun
|e verfasserin
|4 aut
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|a Choi, Nag-Jin
|e verfasserin
|4 aut
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|a Seo, Kang Seok
|e verfasserin
|4 aut
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|a Cho, Young Sik
|e verfasserin
|4 aut
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|a Kim, MinSeok S
|e verfasserin
|4 aut
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|a Ko, Sungho
|e verfasserin
|4 aut
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|a Seo, Jae-Min
|e verfasserin
|4 aut
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|a Lee, Seung-Youp
|e verfasserin
|4 aut
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|a Chae, Jung-Il
|e verfasserin
|4 aut
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|a Lee, Hyun-Jeong
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Asian-Australasian journal of animal sciences
|d 1998
|g 29(2016), 8 vom: 20. Aug., Seite 1197-206
|w (DE-627)NLM098195883
|x 1011-2367
|7 nnns
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| 773 |
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|g volume:29
|g year:2016
|g number:8
|g day:20
|g month:08
|g pages:1197-206
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|u http://dx.doi.org/10.5713/ajas.16.0051
|3 Volltext
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