Plant VAP27 proteins domain characterization, intracellular localization and role in plant development

Plant VAP27 proteins : domain characterization, intracellular localization and role in plant development

© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 210(2016), 4 vom: 26. Juni, Seite 1311-26
1. Verfasser: Wang, Pengwei (VerfasserIn)
Weitere Verfasser: Richardson, Christine, Hawkins, Timothy J, Sparkes, Imogen, Hawes, Chris, Hussey, Patrick J
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article NET superfamily Scs2 VAP27 endoplasmic reticulum (ER) endoplasmic reticulum/plasma membrane (ER/PM) contact sites (EPCSs) Arabidopsis Proteins At3g60600 protein, Arabidopsis Membrane Proteins R-SNARE Proteins
Beschreibung
Zusammenfassung:© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.
The endoplasmic reticulum (ER) is connected to the plasma membrane (PM) through the plant-specific NETWORKED protein, NET3C, and phylogenetically conserved vesicle-associated membrane protein-associated proteins (VAPs). Ten VAP homologues (VAP27-1 to 27-10) can be identified in the Arabidopsis genome and can be divided into three clades. Representative members from each clade were tagged with fluorescent protein and expressed in Nicotiana benthamiana. Proteins from clades I and III localized to the ER as well as to ER/PM contact sites (EPCSs), whereas proteins from clade II were found only at the PM. Some of the VAP27-labelled EPCSs localized to plasmodesmata, and we show that the mobility of VAP27 at EPCSs is influenced by the cell wall. EPCSs closely associate with the cytoskeleton, but their structure is unaffected when the cytoskeleton is removed. VAP27-labelled EPCSs are found in most cell types in Arabidopsis, with the exception of cells in early trichome development. Arabidopsis plants expressing VAP27-GFP fusions exhibit pleiotropic phenotypes, including defects in root hair morphogenesis. A similar effect is also observed in plants expressing VAP27 RNAi. Taken together, these data indicate that VAP27 proteins used at EPCSs are essential for normal ER-cytoskeleton interaction and for plant development
Beschreibung:Date Completed 08.03.2018
Date Revised 13.12.2023
published: Print-Electronic
GENBANK: At1g08820, At1g51270, At2g23820, At2g45140, At3g60600, At4g00170, At4g05060, At4g21450, At5g47180, At5g54110
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.13857