Leaf water oxygen isotope measurement by direct equilibration
© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.
Veröffentlicht in: | The New phytologist. - 1979. - 211(2016), 3 vom: 15. Aug., Seite 1120-8 |
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Format: | Online-Aufsatz |
Sprache: | English |
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2016
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Zugriff auf das übergeordnete Werk: | The New phytologist |
Schlagworte: | Journal Article Research Support, Non-U.S. Gov't isotope analysis isotope ratio mass spectrometer leaf water enrichment oxygen isotope tunable diode laser Oxygen Isotopes Water 059QF0KO0R |
Zusammenfassung: | © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust. The oxygen isotope composition of leaf water imparts a signal to a range of molecules in the atmosphere and biosphere, but has been notoriously difficult to measure in studies requiring a large number of samples as a consequence of the labour-intensive extraction step. We tested a method of direct equilibration of water in fresh leaf samples with CO2 , and subsequent oxygen isotope analysis on an optical spectrometer. The oxygen isotope composition of leaf water measured by the direct equilibration technique was strongly linearly related to that of cryogenically extracted leaf water in paired samples for a wide range of species with differing anatomy, with an R(2) of 0.95. The somewhat more enriched values produced by the direct equilibration method may reflect lack of full equilibration with unenriched water in the vascular bundles, but the strong relationship across a wide range of species suggests that this difference can be adequately corrected for using a simple linear relationship |
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Beschreibung: | Date Completed 30.01.2018 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
ISSN: | 1469-8137 |
DOI: | 10.1111/nph.13962 |