SWAPDT : A method for Short-time Withering Assessment of Probability for Drought Tolerance in Camellia sinensis validated by targeted metabolomics

Copyright © 2016. Published by Elsevier GmbH.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 198(2016) vom: 01. Juli, Seite 39-48
1. Verfasser: Nyarukowa, Christopher (VerfasserIn)
Weitere Verfasser: Koech, Robert, Loots, Theodor, Apostolides, Zeno
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Abiotic stress Amino acids Camellia sinensis Carbohydrates Drought tolerance GC–MS LC–MS Metabolite profiling Short-time withering mehr... Targeted metabolomics Amino Acids Flavonoids Polyphenols Water 059QF0KO0R
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245 1 0 |a SWAPDT  |b A method for Short-time Withering Assessment of Probability for Drought Tolerance in Camellia sinensis validated by targeted metabolomics 
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500 |a Date Revised 30.09.2020 
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520 |a Copyright © 2016. Published by Elsevier GmbH. 
520 |a Climate change is causing droughts affecting crop production on a global scale. Classical breeding and selection strategies for drought-tolerant cultivars will help prevent crop losses. Plant breeders, for all crops, need a simple and reliable method to identify drought-tolerant cultivars, but such a method is missing. Plant metabolism is often disrupted by abiotic stress conditions. To survive drought, plants reconfigure their metabolic pathways. Studies have documented the importance of metabolic regulation, i.e. osmolyte accumulation such as polyols and sugars (mannitol, sorbitol); amino acids (proline) during drought. This study identified and quantified metabolites in drought tolerant and drought susceptible Camellia sinensis cultivars under wet and drought stress conditions. For analyses, GC-MS and LC-MS were employed for metabolomics analysis.%RWC results show how the two drought tolerant and two drought susceptible cultivars differed significantly (p≤0.05) from one another; the drought susceptible exhibited rapid water loss compared to the drought tolerant. There was a significant variation (p<0.05) in metabolite content (amino acid, sugars) between drought tolerant and drought susceptible tea cultivars after short-time withering conditions. These metabolite changes were similar to those seen in other plant species under drought conditions, thus validating this method. The Short-time Withering Assessment of Probability for Drought Tolerance (SWAPDT) method presented here provides an easy method to identify drought tolerant tea cultivars that will mitigate the effects of drought due to climate change on crop losses 
650 4 |a Journal Article 
650 4 |a Abiotic stress 
650 4 |a Amino acids 
650 4 |a Camellia sinensis 
650 4 |a Carbohydrates 
650 4 |a Drought tolerance 
650 4 |a GC–MS 
650 4 |a LC–MS 
650 4 |a Metabolite profiling 
650 4 |a Short-time withering 
650 4 |a Targeted metabolomics 
650 7 |a Amino Acids  |2 NLM 
650 7 |a Flavonoids  |2 NLM 
650 7 |a Polyphenols  |2 NLM 
650 7 |a Water  |2 NLM 
650 7 |a 059QF0KO0R  |2 NLM 
700 1 |a Koech, Robert  |e verfasserin  |4 aut 
700 1 |a Loots, Theodor  |e verfasserin  |4 aut 
700 1 |a Apostolides, Zeno  |e verfasserin  |4 aut 
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