|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM259714046 |
| 003 |
DE-627 |
| 005 |
20240114232652.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2016 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1111/nph.13964
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1253.xml
|
| 035 |
|
|
|a (DE-627)NLM259714046
|
| 035 |
|
|
|a (NLM)27111838
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Flis, Paulina
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Inventory of metal complexes circulating in plant fluids
|b a reliable method based on HPLC coupled with dual elemental and high-resolution molecular mass spectrometric detection
|
| 264 |
|
1 |
|c 2016
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 30.01.2018
|
| 500 |
|
|
|a Date Revised 09.01.2024
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.
|
| 520 |
|
|
|a Description of metal species in plant fluids such as xylem, phloem or related saps remains a complex challenge usually addressed either by liquid chromatography-mass spectrometry, X-ray analysis or computational prediction. To date, none of these techniques has achieved a complete and true picture of metal-containing species in plant fluids, especially for the least concentrated complexes. Here, we present a generic analytical methodology for a large-scale (> 10 metals, > 50 metal complexes) detection, identification and semiquantitative determination of metal complexes in the xylem and embryo sac liquid of the green pea, Pisum sativum. The procedure is based on direct injection using hydrophilic interaction chromatography with dual detection by elemental (inductively coupled plasma mass spectrometry) and molecular (high-resolution electrospray mass spectrometry) mass spectrometric detection. Numerous and novel complexes of iron(II), iron(III), copper(II), zinc, manganese, cobalt(II), cobalt(III), magnesium, calcium, nickel and molybdenum(IV) with several ligands including nicotianamine, citrate, malate, histidine, glutamine, aspartic acid, asparagine, phenylalanine and others are observed in pea fluids and discussed. This methodology provides a large inventory of various types of metal complexes, which is a significant asset for future biochemical and genetic studies into metal transport/homeostasis
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Pisum sativum
|
| 650 |
|
4 |
|a embryo sac liquid
|
| 650 |
|
4 |
|a hydrophilic interaction chromatography (HILIC)
|
| 650 |
|
4 |
|a mass spectrometry
|
| 650 |
|
4 |
|a metal complexes
|
| 650 |
|
4 |
|a metal speciation
|
| 650 |
|
4 |
|a nicotianamine (NA)
|
| 650 |
|
4 |
|a xylem
|
| 650 |
|
7 |
|a Amino Acids
|2 NLM
|
| 650 |
|
7 |
|a Carboxylic Acids
|2 NLM
|
| 650 |
|
7 |
|a Coordination Complexes
|2 NLM
|
| 650 |
|
7 |
|a Metals
|2 NLM
|
| 700 |
1 |
|
|a Ouerdane, Laurent
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Grillet, Louis
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Curie, Catherine
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Mari, Stéphane
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Lobinski, Ryszard
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 211(2016), 3 vom: 22. Aug., Seite 1129-41
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
|
| 773 |
1 |
8 |
|g volume:211
|g year:2016
|g number:3
|g day:22
|g month:08
|g pages:1129-41
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/nph.13964
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 211
|j 2016
|e 3
|b 22
|c 08
|h 1129-41
|