Convergence of CONSTITUTIVE PHOTOMORPHOGENESIS 1 and PHYTOCHROME INTERACTING FACTOR signalling during shade avoidance

© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1990. - 211(2016), 3 vom: 21. Aug., Seite 967-79
1. Verfasser: Pacín, Manuel (VerfasserIn)
Weitere Verfasser: Semmoloni, Mariana, Legris, Martina, Finlayson, Scott A, Casal, Jorge J
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Arabidopsis thaliana CONSTITUTIVE PHOTOMORPHOGENESIS 1 (COP1) ELONGATED HYPOCOTYL 5 (HY5) LONG HYPOCOTYL IN FAR-RED (HFR1) PHYTOCHROME INTERACTING FACTOR (PIF) auxin shade avoidance Arabidopsis Proteins Basic Helix-Loop-Helix Transcription Factors mehr... Indoleacetic Acids AT2G32950 protein, Arabidopsis EC 2.3.2.27 Ubiquitin-Protein Ligases
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520 |a Shade-avoidance responses require CONSTITUTIVE PHOTOMORPHOGENESIS 1 (COP1) but the mechanisms of action of COP1 under shade have not been elucidated. Using simulated shade and control conditions, we analysed: the transcriptome and the auxin levels of cop1 and phytochrome interacting factor 1 (pif1) pif3 pif4 pif5 (pifq) mutants; the dynamics of ELONGATED HYPOCOTYL 5 (HY5) and LONG HYPOCOTYL IN FAR-RED (HFR1) proteins; and the epistatic relationships between cop1 and pif3, pif4, pif5, hy5 and hfr1 mutations in Arabidopsis thaliana. Despite severely impaired shade-avoidance responses, only a few genes that responded to shade in the wild-type failed to do so in cop1. Shade enhanced the convergence between cop1 and pifq transcriptomes, mainly on shade-avoidance marker genes. Shade failed to increase auxin levels in cop1. Residual shade avoidance in cop1 was not further reduced by the pif3, pif4 or pif5 mutations, suggesting convergent pathways. HFR1 stability decreased under shade in a COP1-dependent manner but shade increased HY5 stability. The cop1 mutant retains responses to shade and is more specifically impaired in shade avoidance. COP1 promotes the degradation of HFR1 under shade, thus increasing the ability of PIFs to control gene expression, increase auxin levels and promote stem growth 
650 4 |a Journal Article 
650 4 |a Arabidopsis thaliana 
650 4 |a CONSTITUTIVE PHOTOMORPHOGENESIS 1 (COP1) 
650 4 |a ELONGATED HYPOCOTYL 5 (HY5) 
650 4 |a LONG HYPOCOTYL IN FAR-RED (HFR1) 
650 4 |a PHYTOCHROME INTERACTING FACTOR (PIF) 
650 4 |a auxin 
650 4 |a shade avoidance 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a Basic Helix-Loop-Helix Transcription Factors  |2 NLM 
650 7 |a Indoleacetic Acids  |2 NLM 
650 7 |a AT2G32950 protein, Arabidopsis  |2 NLM 
650 7 |a EC 2.3.2.27  |2 NLM 
650 7 |a Ubiquitin-Protein Ligases  |2 NLM 
650 7 |a EC 2.3.2.27  |2 NLM 
700 1 |a Semmoloni, Mariana  |e verfasserin  |4 aut 
700 1 |a Legris, Martina  |e verfasserin  |4 aut 
700 1 |a Finlayson, Scott A  |e verfasserin  |4 aut 
700 1 |a Casal, Jorge J  |e verfasserin  |4 aut 
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