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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erw146
|2 doi
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|a pubmed25n0865.xml
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|a (NLM)27091877
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Guo, Kai
|e verfasserin
|4 aut
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| 245 |
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|a Fibre elongation requires normal redox homeostasis modulated by cytosolic ascorbate peroxidase in cotton (Gossypium hirsutum)
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 20.12.2017
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|a Date Revised 25.03.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a High-quality cotton fibre equates to a more comfortable textile. Fibre length is an important index of fibre quality. Hydrogen peroxide (H2O2) acts as a signalling molecule in the regulation of fibre elongation. Results from in vitro ovule culture suggest that the alteration of fibre cell H2O2 levels affects fibre development. Ascorbate peroxidase (APX) is an important reactive oxygen species (ROS) scavenging enzyme, and we found that GhAPX1AT/DT encoded one member of the previously unrealized group of cytosolic APXs (cAPXs) that were preferentially expressed during the fibre elongation stage. Transgenic cottons with up- and down-regulation of GhAPX1AT/DT were generated to control fibre endogenous levels of H2O2 Suppression of all cAPX (IAO) resulted in a 3.5-fold increase in H2O2 level in fibres and oxidative stress, which significantly suppressed fibre elongation. The fibre length of transgenic lines with over-expression or specific down-regulation of GhAPX1AT/DT did not show any obvious change. However, the fibres in the over-expression lines exhibited higher tolerance to oxidative stress. Differentially expressed genes (DEGs) in fibres at 10 days post-anthesis (DPA) of IAO lines identified by RNA-seq were related to redox homeostasis, signalling pathways, stress responses and cell wall synthesis, and the DEGs that were up-regulated in IAO lines were also up-regulated in the 10 DPA and 20 DPA fibres of wild cotton compared with domesticated cotton. These results suggest that optimal H2O2 levels and redox state regulated by cytosolic APX are key mechanisms regulating fibre elongation, and dysregulation of the increase in H2O2 induces oxidative stress and results in shorter fibres by initiating secondary cell wall-related gene expression
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cytosolic ascorbate peroxidase
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|a Gossypium hirsutum
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|a fibre elongation
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|a hydrogen peroxide
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|a oxidative stress
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|a redox homeostasis.
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|a Plant Proteins
|2 NLM
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|a Hydrogen Peroxide
|2 NLM
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|a BBX060AN9V
|2 NLM
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|a Ascorbate Peroxidases
|2 NLM
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| 650 |
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|a EC 1.11.1.11
|2 NLM
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| 700 |
1 |
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|a Du, Xueqiong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tu, Lili
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tang, Wenxin
|e verfasserin
|4 aut
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| 700 |
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|a Wang, Pengcheng
|e verfasserin
|4 aut
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| 700 |
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|a Wang, Maojun
|e verfasserin
|4 aut
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|a Liu, Zhen
|e verfasserin
|4 aut
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| 700 |
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|a Zhang, Xianlong
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 67(2016), 11 vom: 09. Mai, Seite 3289-301
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnas
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| 773 |
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|g volume:67
|g year:2016
|g number:11
|g day:09
|g month:05
|g pages:3289-301
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erw146
|3 Volltext
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