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231224s2016 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.2144/000114402
|2 doi
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|a pubmed25n0864.xml
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|a (DE-627)NLM259338575
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|a (NLM)27071607
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Afiuni-Zadeh, Somaieh
|e verfasserin
|4 aut
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| 245 |
1 |
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|a AminoxyTMT
|b A novel multi-functional reagent for characterization of protein carbonylation
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|c 2016
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 28.12.2016
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|a Date Revised 30.12.2016
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|a published: Electronic-eCollection
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|a Citation Status MEDLINE
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|a Protein carbonylation is a common oxidative stress (OS)-driven post-translational modification (PTM). Proteome-wide carbonylation events can best be characterized using a combination of analytical approaches. Immunoblotting of carbonylated proteins provides data on the extent of modifications within complex samples, as well as a broad comparison of carbonylation profiles between different biological states (e.g., disease versus control), while mass spectrometry (MS)-based analysis provides information on proteins susceptible to carbonylation, as well as the potential for quantitative characterization of specific sites of amino acid modification. Here, we present a novel use for aminoxyTMT, a derivative of the Tandem Mass Tag (TMT) isobaric labeling reagent, which utilizes an aminooxy functional group for covalent labeling of reactive carbonyls in proteins. When coupled with anti-TMT antibody, we demonstrate the use of aminoxyTMT for immunoblot profiling of protein carbonylation in complex mixtures, as well as enrichment of modified peptides from these mixtures. Proof-of-principle experiments also show the amenability of aminoxyTMT-labeled carbonylated peptides enriched from complex mixtures to identification using tandem MS (MS/MS) and database searching, as well as quantitative analysis using TMT-based reporter ion intensity measurements
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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4 |
|a AminoxyTMT
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|a Immunoblotting
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|a Mass spectrometry
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|a Post translational modification
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| 650 |
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|a Protein carbonylation
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|a Tandem Mass Tag
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| 650 |
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|a Indicators and Reagents
|2 NLM
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| 650 |
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|a Peptides
|2 NLM
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| 650 |
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|a Proteins
|2 NLM
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| 700 |
1 |
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|a Rogers, John C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Snovida, Sergei I
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bomgarden, Ryan D
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Griffin, Timothy J
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 60(2016), 4 vom: 14. Apr., Seite 186-8, 190, 192-6
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
8 |
|g volume:60
|g year:2016
|g number:4
|g day:14
|g month:04
|g pages:186-8, 190, 192-6
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|u http://dx.doi.org/10.2144/000114402
|3 Volltext
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