Mutant DNA quantification by digital PCR can be confounded by heating during DNA fragmentation
Digital PCR (dPCR) is gaining popularity as a DNA mutation quantification method for clinical specimens. Fragmentation prior to dPCR is required for non-fragmented genomic DNA samples; however, the effect of fragmentation on DNA analysis has not been well-studied. Here we evaluated three fragmentati...
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Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 60(2016), 4 vom: 14. Apr., Seite 175-6, 178, 180 passim
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| 1. Verfasser: |
Kang, Qing
(VerfasserIn) |
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| Weitere Verfasser: |
Parkin, Brian,
Giraldez, Maria D,
Tewari, Muneesh |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2016
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
DNA mutation
droplet digital PCR
fragmentation
heating
KRAS protein, human
DNA
9007-49-2
BRAF protein, human
mehr...
EC 2.7.11.1
Proto-Oncogene Proteins B-raf
Proto-Oncogene Proteins p21(ras)
EC 3.6.5.2 |
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