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231224s2016 xx |||||o 00| ||eng c |
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|a 10.3928/23258160-20160324-07
|2 doi
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|a pubmed25n0864.xml
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|e rakwb
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|a eng
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|a Brant Fernandes, Rodrigo A
|e verfasserin
|4 aut
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|a An Innovative Surgical Technique for Subretinal Transplantation of Human Embryonic Stem Cell-Derived Retinal Pigmented Epithelium in Yucatan Mini Pigs
|b Preliminary Results
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 13.12.2016
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|a Date Revised 30.12.2016
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|a published: Print
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|a Citation Status MEDLINE
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|a Copyright 2016, SLACK Incorporated.
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|a BACKGROUND AND OBJECTIVE: To develop a safe and efficient surgical procedure for subretinal implantation into porcine eyes of a human embryonic stem cell-derived retinal pigmented epithelium (hESC-RPE) monolayer seeded onto a Parylene-C scaffold. This implant is referred to as CPCB-RPE1
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|a MATERIALS AND METHODS: Ultrathin Parylene-C scaffolds were seeded with hESC-RPE and surgically implanted into the subretinal space of Yucatan mini pigs (n = 8). The surgery consisted of pars plana vitrectomy, induction of a limited retinal detachment, and peripheral retinotomy for insertion of the monolayer using a novel tissue injector, followed by silicone oil tamponade injection, laser photocoagulation around the retinotomy site, and inferior iridectomy. Oral cyclosporine was administered from day 1 and during the entire follow-up period. Three months later, the animals were euthanized and the eyes and major organs were submitted for histological analysis. Adjacent sections underwent immunohistochemical analysis to detect human cells using anti-TRA-1-85 (human blood group antigen) antibody and DAPI antibodies
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|a RESULTS: The cell monolayer was immunopositive for TRA-1-85 3 months after implantation and migration from the Parylene-C scaffold was not detected. One eye had a mild inflammatory reaction around the implant that was negative for human biomarkers. No intraocular or systemic tumors were detected
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|a CONCLUSION: The hESC-RPE cells survived for 3 months in this animal model. The surgical procedure for subretinal implantation of CPCB-RPE1 is feasible and safe, without cell migration off the scaffold or development of ocular or systemic tumors
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Polymers
|2 NLM
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|a Xylenes
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|a parylene
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|a 25722-33-2
|2 NLM
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|a Koss, Michael Janusz
|e verfasserin
|4 aut
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|a Falabella, Paulo
|e verfasserin
|4 aut
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| 700 |
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|a Stefanini, Francisco Rosa
|e verfasserin
|4 aut
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|a Maia, Mauricio
|e verfasserin
|4 aut
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|a Diniz, Bruno
|e verfasserin
|4 aut
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|a Ribeiro, Ramiro
|e verfasserin
|4 aut
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|a Hu, Yuntao
|e verfasserin
|4 aut
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|a Hinton, David
|e verfasserin
|4 aut
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|a Clegg, Dennis O
|e verfasserin
|4 aut
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|a Chader, Gerald
|e verfasserin
|4 aut
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|a Humayun, Mark S
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Ophthalmic surgery, lasers & imaging retina
|d 2013
|g 47(2016), 4 vom: 01. Apr., Seite 342-51
|w (DE-627)NLM224956647
|x 2325-8179
|7 nnns
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| 773 |
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|g volume:47
|g year:2016
|g number:4
|g day:01
|g month:04
|g pages:342-51
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