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231224s2008 xx |||||o 00| ||eng c |
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|a 10.1111/j.1529-8817.2008.00575.x
|2 doi
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|a eng
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1 |
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|a Contreras, Loretto
|e verfasserin
|4 aut
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| 245 |
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|a TWO-DIMENSIONAL GEL ELECTROPHORESIS ANALYSIS OF BROWN ALGAL PROTEIN EXTRACTS(1)
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|c 2008
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 29.04.2016
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|a Date Revised 10.03.2022
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|a published: Print-Electronic
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|a GENBANK: AAF66150, AAL47754, AAL76325, AAL79536, AAM93951, AAN62452, AAS55634, AAU11484, ABB02445.1, CAC67484, CAI56206
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|a Citation Status PubMed-not-MEDLINE
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|a © 2008 Phycological Society of America.
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|a High-quality protein extracts are required for proteomic studies, a field that is poorly developed for marine macroalgae. A reliable phenol extraction protocol using Scytosiphon gracilis Kogame and Ectocarpus siliculosus (Dillwyn) Lyngb. (Phaeophyceae) as algal models resulted in high-quality protein extracts. The performance of the new protocol was tested against four methods available for vascular plants and a seaweed. The protocol, which includes an initial step to remove salts from the algal tissues, allowed the use of highly resolving two-dimensional gel electrophoresis (2-DE) protein analyses, providing the opportunity to unravel potentially novel physiological processes unique to this group of marine organisms
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|a Journal Article
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|a 2-DE
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|a Phaeophyceae
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|a algae
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|a protein extraction methods
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|a proteomics
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|a Ritter, Andrés
|e verfasserin
|4 aut
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|a Dennett, Geraldine
|e verfasserin
|4 aut
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|a Boehmwald, Freddy
|e verfasserin
|4 aut
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|a Guitton, Nathalie
|e verfasserin
|4 aut
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|a Pineau, Charles
|e verfasserin
|4 aut
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|a Moenne, Alejandra
|e verfasserin
|4 aut
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| 700 |
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|a Potin, Philippe
|e verfasserin
|4 aut
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| 700 |
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|a Correa, Juan A
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of phycology
|d 1966
|g 44(2008), 5 vom: 04. Okt., Seite 1315-21
|w (DE-627)NLM098182994
|x 1529-8817
|7 nnns
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|g volume:44
|g year:2008
|g number:5
|g day:04
|g month:10
|g pages:1315-21
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