UNDERSTANDING NITROGEN LIMITATION IN AUREOCOCCUS ANOPHAGEFFERENS (PELAGOPHYCEAE) THROUGH cDNA AND qRT-PCR ANALYSIS(1)
© 2008 Phycological Society of America.
| Veröffentlicht in: | Journal of phycology. - 1966. - 44(2008), 5 vom: 04. Okt., Seite 1235-49 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2008
|
| Zugriff auf das übergeordnete Werk: | Journal of phycology |
| Schlagworte: | Journal Article Aureococcus anophagefferens amino acid/polyamine transporter ammonium transporter formate/nitrite transporter gene expression nitrate transporter nitrogen limitation picoeukaryote purine transporter |
| Zusammenfassung: | © 2008 Phycological Society of America. Brown tides of the marine pelagophyte Aureococcus anophagefferens Hargraves et Sieburth have been investigated extensively for the past two decades. Its growth is fueled by a variety of nitrogen (N) compounds, with dissolved organic nitrogen (DON) being particularly important during blooms. Characterization of a cDNA library suggests that A. anophagefferens can assimilate eight different forms of N. Expression of genes related to the sensing, uptake, and assimilation of inorganic and organic N, as well as the catabolic process of autophagy, was assayed in cells grown on different N sources and in N-limited cells. Growth on nitrate elicited an increase in the relative expression of nitrate and ammonium transporters, a nutrient stress-induced transporter, and a sensory kinase. Growth on urea increased the relative expression of a urea and a formate/nitrite transporter, while growth on ammonium resulted in an increase in the relative expression of an ammonium transporter, a novel ATP-binding cassette (ABC) transporter and a putative high-affinity phosphate transporter. N limitation resulted in a 30- to 110-fold increase in the relative expression of nitrate, ammonium, urea, amino acid/polyamine, and formate/nitrite transporters. A. anophagefferens demonstrated the highest relative accumulation of a transcript encoding a novel purine transporter, which was highly expressed across all N sources. This finding suggests that purines are an important source of N for the growth of this organism and could possibly contribute to the initiation and maintenance of blooms in the natural environment |
|---|---|
| Beschreibung: | Date Completed 29.04.2016 Date Revised 04.04.2016 published: Print-Electronic GENBANK: AAD18085, AAI51200, AAK52491, AAL85928, AAM98105, AAO47330, AAS00548, AAS54154, AAS89990, AAT39458, AAV70490, AAV98625, AAX96020, AAY59538, ABB47779, ABC80592, ABK15648, ABO94523, ABO94771, AX460946, BAB92527, BAD09633, BAF05798, BAF06260, BAF44666, CAB40167, CAC43845, CAI72310, CAL56669, EAA30029, EAL65439, EAL70835, EAQ78033, EAU35126, EAW64094, EAX52986, EAX99999, EAY72693, EAZ07291, EDN45381, EH058498, EH058499, EH058500, EH058501, EH058515, EH058526, EH058527, EH058528, EH058529, EH058530, EH058531, EH058532, EH058533, EH058535, EH058536, EH058537, EH058538, EH058539, EH058540, EH058541, EH058542, EH058543, EH058544, EH058545, EH117318, EH117748, EH117765, EH412134, EH412633, EH58546, EH668998, EH669232, EV780853, EV780854, EV780855, EV780856, EV780857, EV780858, EV780859, EV780860, EV780861, EV780862, EV780863, EV780864, EV780865, EV780866, EV780867, EV780868, EV780869, EV780870, EV780871, EV780872, EV780873, EV780874, EV780875, EV780876, EV789876, NP778169, PF00860, Q8H715, XP1215712, XP1653235, XP657829, XP685369, XP786368 Citation Status PubMed-not-MEDLINE |
| ISSN: | 1529-8817 |
| DOI: | 10.1111/j.1529-8817.2008.00571.x |