|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM259016543 |
| 003 |
DE-627 |
| 005 |
20250219224456.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2016 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.plaphy.2016.03.033
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n0863.xml
|
| 035 |
|
|
|a (DE-627)NLM259016543
|
| 035 |
|
|
|a (NLM)27038601
|
| 035 |
|
|
|a (PII)S0981-9428(16)30102-4
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Nguyen, Van Phap
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Evaluation of a novel promoter from Populus trichocarpa for mature xylem tissue specific gene delivery
|
| 264 |
|
1 |
|c 2016
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 27.03.2017
|
| 500 |
|
|
|a Date Revised 30.09.2020
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2016 Elsevier Masson SAS. All rights reserved.
|
| 520 |
|
|
|a Wood (i.e., secondary xylem) is an important raw material for many industrial applications. Mature xylem (MX) tissue-specific genetic modification offers an effective means to improve the chemical and physical properties of the wood. Here, we describe a promoter that drives strong gene expression in a MX tissue-specific manner. Using whole-transcriptome genechip analyses of different tissue types of poplar, we identified five candidate genes that had strong expression in the MX tissue. The putative promoter sequences of the five MX-specific genes were evaluated for their promoter activity in both transgenic Arabidopsis and poplar. Among them, we found the promoter of Potri.013G007900.1 (called the PtrMX3 promoter) had the strongest activity in MX and thus was further characterized. In the stem and root tissues of transgenic Arabidopsis plants, the PtrMX3 promoter activity was found exclusively in MX tissue. MX-specific activity of the promoter was reproduced in the stem tissue of transgenic poplar plants. The PtrMX3 promoter activity was not influenced by abiotic stresses or exogenously applied growth regulators, indicating the PtrMX3 promoter is bona fide MX tissue-specific. Our study provides a strong MX-specific promoter for MX-specific modifications of woody biomass
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Biotechnology
|
| 650 |
|
4 |
|a Mature xylem
|
| 650 |
|
4 |
|a Poplar
|
| 650 |
|
4 |
|a Tissue-specific promoter
|
| 650 |
|
4 |
|a Woody biomass
|
| 650 |
|
7 |
|a Plant Growth Regulators
|2 NLM
|
| 650 |
|
7 |
|a Glucuronidase
|2 NLM
|
| 650 |
|
7 |
|a EC 3.2.1.31
|2 NLM
|
| 700 |
1 |
|
|a Cho, Jin-Seong
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Choi, Young-Im
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Lee, Sang-Won
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Han, Kyung-Hwan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Ko, Jae-Heung
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 104(2016) vom: 05. Juli, Seite 226-33
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnas
|
| 773 |
1 |
8 |
|g volume:104
|g year:2016
|g day:05
|g month:07
|g pages:226-33
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.plaphy.2016.03.033
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 104
|j 2016
|b 05
|c 07
|h 226-33
|