FIRST INDUCED PLASTID GENOME MUTATIONS IN AN ALGA WITH SECONDARY PLASTIDS psbA MUTATIONS IN THE DIATOM PHAEODACTYLUM TRICORNUTUM (BACILLARIOPHYCEAE) REVEAL CONSEQUENCES ON THE REGULATION OF PHOTOSYNTHESIS(1)

FIRST INDUCED PLASTID GENOME MUTATIONS IN AN ALGA WITH SECONDARY PLASTIDS : psbA MUTATIONS IN THE DIATOM PHAEODACTYLUM TRICORNUTUM (BACILLARIOPHYCEAE) REVEAL CONSEQUENCES ON THE REGULATION OF PHOTOSYNTHESIS(1)

© 2009 Phycological Society of America.

Bibliographische Detailangaben
Veröffentlicht in:Journal of phycology. - 1966. - 45(2009), 4 vom: 01. Aug., Seite 838-46
1. Verfasser: Materna, Arne C (VerfasserIn)
Weitere Verfasser: Sturm, Sabine, Kroth, Peter G, Lavaud, Johann
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:Journal of phycology
Schlagworte:Journal Article D1 protein QB pocket chlorophyll fluorescence diatom electron transport herbicide photosystem II
Beschreibung
Zusammenfassung:© 2009 Phycological Society of America.
Diatoms play a crucial role in the biochemistry and ecology of most aquatic ecosystems, especially because of their high photosynthetic productivity. They often have to cope with a fluctuating light climate and a punctuated exposure to excess light, which can be harmful for photosynthesis. To gain insight into the regulation of photosynthesis in diatoms, we generated and studied mutants of the diatom Phaeodactylum tricornutum Bohlin carrying functionally altered versions of the plastidic psbA gene encoding the D1 protein of the PSII reaction center (PSII RC). All analyzed mutants feature an amino acid substitution in the vicinity of the QB -binding pocket of D1. We characterized the photosynthetic capacity of the mutants in comparison to wildtype cells, focusing on the way they regulate their photochemistry as a function of light intensity. The results show that the mutations resulted in constitutive changes of PSII electron transport rates. The extent of the impairment varies between mutants depending on the proximity of the mutation to the QB -binding pocket and/or to the nonheme iron within the PSII RC. The effects of the mutations described here for P. tricornutum are similar to effects in cyanobacteria and green microalgae, emphasizing the conservation of the D1 protein structure among photosynthetic organisms of different evolutionary origins
Beschreibung:Date Completed 04.04.2016
Date Revised 01.04.2016
published: Print
GENBANK: AY864816
Citation Status PubMed-not-MEDLINE
ISSN:1529-8817
DOI:10.1111/j.1529-8817.2009.00711.x