LOCALIZATION OF MICROCYSTIN SYNTHETASE GENES IN COLONIES OF THE CYANOBACTERIUM MICROCYSTIS USING FLUORESCENCE IN SITU HYBRIDIZATION(1)
© 2009 Phycological Society of America.
| Veröffentlicht in: | Journal of phycology. - 1966. - 45(2009), 6 vom: 01. Dez., Seite 1400-4 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2009
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| Zugriff auf das übergeordnete Werk: | Journal of phycology |
| Schlagworte: | Journal Article DNA ELISA Microcystis PCR fluorescence in situ hybridization microcystin |
| Zusammenfassung: | © 2009 Phycological Society of America. To better understand the production of microcystins (MCs) in Microcystis colonies, fluorescence in situ hybridization (FISH) methods were developed to detect DNA involved in the synthesis of these cyanobacterial hepatotoxins. Using colonies of Microcystis aeruginosa (Kütz.) Kütz. isolated from environmental blooms of cyanobacteria and from a colony-forming, MC-producing laboratory strain of Microcystis, amplified PCR products were observed, coincident with positive controls. The total MC content of individual colonies of Microcystis, determined by ELISA, showed a positive correlation with colony cross-sectional area. FISH analysis of Microcystis colonies gave high fluorescence in comparison to negative controls, indicating the presence of MC synthetase DNA (mcyA) in situ. FISH analysis for MC synthetase genes has the potential to be developed into an effective early warning tool for drinking and recreational water management |
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| Beschreibung: | Date Completed 04.04.2016 Date Revised 01.04.2016 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
| ISSN: | 1529-8817 |
| DOI: | 10.1111/j.1529-8817.2009.00750.x |