Specific and Reversible DNA-Directed Self-Assembly of Modular Vesicle-Droplet Hybrid Materials

Modular hybrid structures functionalized to assemble in a controlled manner possess diverse properties necessary for a new generation of complex materials and applications. Here, we functionalized giant unilamellar vesicles and emulsion droplets with biotinylated single-stranded DNA oligonucleotides...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 32(2016), 15 vom: 19. Apr., Seite 3561-6
1. Verfasser: Hadorn, Maik (VerfasserIn)
Weitere Verfasser: Boenzli, Eva, Hanczyc, Martin M
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Letter Research Support, Non-U.S. Gov't 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000) DNA, Single-Stranded Indicators and Reagents Membranes, Artificial Phosphatidylcholines Phosphatidylethanolamines Unilamellar Liposomes Polyethylene Glycols mehr... 3WJQ0SDW1A Biotin 6SO6U10H04 Streptavidin 9013-20-1 1-palmitoyl-2-oleoylphosphatidylcholine TE895536Y5
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520 |a Modular hybrid structures functionalized to assemble in a controlled manner possess diverse properties necessary for a new generation of complex materials and applications. Here, we functionalized giant unilamellar vesicles and emulsion droplets with biotinylated single-stranded DNA oligonucleotides using streptavidin as an intermediary linker to demonstrate specific and reversible DNA-directed self-assembly into vesicle-droplet hybrid structures. A low molar percentage of PEGylated phospholipids independent of the DNA-based recognition machinery at the supramolecular surface modulated the stability of the system. The reversibility of the aggregation was demonstrated by heating the hybrid structures above the melting temperature of the conjoining double-stranded DNA in the presence of excess biotin. The application of this general assembly control system to diverse multiphase soft materials provides the mechanism to assemble complex modular hybrid systems in a controllable and reversible way, which may provide an advantage where multifunctionality is a target property 
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650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Membranes, Artificial  |2 NLM 
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650 7 |a Phosphatidylethanolamines  |2 NLM 
650 7 |a Unilamellar Liposomes  |2 NLM 
650 7 |a Polyethylene Glycols  |2 NLM 
650 7 |a 3WJQ0SDW1A  |2 NLM 
650 7 |a Biotin  |2 NLM 
650 7 |a 6SO6U10H04  |2 NLM 
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650 7 |a 1-palmitoyl-2-oleoylphosphatidylcholine  |2 NLM 
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700 1 |a Boenzli, Eva  |e verfasserin  |4 aut 
700 1 |a Hanczyc, Martin M  |e verfasserin  |4 aut 
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