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231224s2014 xx |||||o 00| ||eng c |
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|a 10.1111/jpy.12187
|2 doi
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|a pubmed25n0861.xml
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|a (DE-627)NLM258530499
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|a (NLM)26988329
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|a DE-627
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|e rakwb
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|a eng
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|a Simioni, Carmen
|e verfasserin
|4 aut
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|a Effects of brefeldin A on the endomembrane system and germ tube formation of the tetraspore of Gelidium floridanum (Rhodophyta, Florideophyceae)
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|c 2014
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 26.07.2016
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|a Date Revised 18.03.2016
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a © 2014 Phycological Society of America.
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|a Gelidium floridanum W.R. Taylor tetraspores are units of dispersal and are responsible for substrate attachment. This study aimed to examine evidence of direct interaction between germ tube formation and Golgi activity during tetraspore germination of G. floridanum. After release, the tetraspores were incubated with brefeldin A (BFA) in concentrations of 4 and 8 μM over a 6 h period. The controls and treatments were analyzed with light, fluorescence (FM4-64 dye) and transmission electron microscopy. In the control samples, the Golgi bodies were responsible for germ tube formation. In contrast, BFA-treated samples were observed to inhibit spore adhesion and germ tube formation. These tetraspores also showed an increase in volume (≥30 μm width). BFA treatment also resulted in the disassembly of Golgi cisternae and the formation of vesiculated areas of the cytoplasm, blocking the secretion of protein and amorphous matrix polysaccharides. When stained with FM4-64, the control samples showed fluorescence in the apical region of the germ tube, but the treated samples showed an intense fluorescence throughout the cytoplasm. From these results, we can conclude that the germ tube is formed by the incorporation of vesicles derived from Golgi. Thus, vesicle secretion and Golgi organization are basic processes and essential in adhesion and tube formation. By blocking the secretion of protein and amorphous matrix polysaccharides, BFA treatment precluded tetraspore germination
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|a Journal Article
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|a Gelidium floridanum
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|a Golgi bodies
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|a brefeldin A
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|a electron microscopy
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|a tetraspore germination
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|a Rover, Ticiane
|e verfasserin
|4 aut
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|a Schmidt, Éder C
|e verfasserin
|4 aut
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|a de L Felix, Marthiellen R
|e verfasserin
|4 aut
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|a Polo, Luz Karime
|e verfasserin
|4 aut
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|a Santos, Rodrigo Dos
|e verfasserin
|4 aut
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| 700 |
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|a Costa, Giulia Burle
|e verfasserin
|4 aut
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| 700 |
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|a Kreusch, Marianne
|e verfasserin
|4 aut
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|a Pereira, Debora T
|e verfasserin
|4 aut
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|a Ouriques, Luciane C
|e verfasserin
|4 aut
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|a Bouzon, Zenilda L
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of phycology
|d 1966
|g 50(2014), 3 vom: 18. Juni, Seite 577-86
|w (DE-627)NLM098182994
|x 0022-3646
|7 nnns
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|g volume:50
|g year:2014
|g number:3
|g day:18
|g month:06
|g pages:577-86
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|u http://dx.doi.org/10.1111/jpy.12187
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