Rice PROTEIN l-ISOASPARTYL METHYLTRANSFERASE isoforms differentially accumulate during seed maturation to restrict deleterious isoAsp and reactive oxygen species accumulation and are implicated in seed vigor and longevity

Rice PROTEIN l-ISOASPARTYL METHYLTRANSFERASE isoforms differentially accumulate during seed maturation to restrict deleterious isoAsp and reactive oxygen species accumulation and are implicated in seed vigor and longevity

© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 211(2016), 2 vom: 29. Juli, Seite 627-45
1. Verfasser: Petla, Bhanu Prakash (VerfasserIn)
Weitere Verfasser: Kamble, Nitin Uttam, Kumar, Meenu, Verma, Pooja, Ghosh, Shraboni, Singh, Ajeet, Rao, Venkateswara, Salvi, Prafull, Kaur, Harmeet, Saxena, Saurabh Chandra, Majee, Manoj
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Oryza sativa PROTEIN l-ISOASPARTYL O-METHYLTRANSFERASE (PIMT) aging isoaspartate longevity repair seed desiccation Isoenzymes mehr... Plant Proteins RNA, Messenger Reactive Oxygen Species Aspartic Acid 30KYC7MIAI Protein D-Aspartate-L-Isoaspartate Methyltransferase EC 2.1.1.77
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245 1 0 |a Rice PROTEIN l-ISOASPARTYL METHYLTRANSFERASE isoforms differentially accumulate during seed maturation to restrict deleterious isoAsp and reactive oxygen species accumulation and are implicated in seed vigor and longevity 
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520 |a © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust. 
520 |a PROTEIN l-ISOASPARTYL O-METHYLTRANSFERASE (PIMT) is a protein-repairing enzyme involved in seed vigor and longevity. However, the regulation of PIMT isoforms during seed development and the mechanism of PIMT-mediated improvement of seed vigor and longevity are largely unknown. In this study in rice (Oryza sativa), we demonstrate the dynamics and correlation of isoaspartyl (isoAsp)-repairing demands and PIMT activity, and their implications, during seed development, germination and aging, through biochemical, molecular and genetic studies. Molecular and biochemical analyses revealed that rice possesses various biochemically active and inactive PIMT isoforms. Transcript and western blot analyses clearly showed the seed development stage and tissue-specific accumulation of active isoforms. Immunolocalization studies revealed distinct isoform expression in embryo and aleurone layers. Further analyses of transgenic lines for each OsPIMT isoform revealed a clear role in the restriction of deleterious isoAsp and age-induced reactive oxygen species (ROS) accumulation to improve seed vigor and longevity. Collectively, our data suggest that a PIMT-mediated, protein repair mechanism is initiated during seed development in rice, with each isoform playing a distinct, yet coordinated, role. Our results also raise the intriguing possibility that PIMT repairs antioxidative enzymes and proteins which restrict ROS accumulation, lipid peroxidation, etc. in seed, particularly during aging, thus contributing to seed vigor and longevity 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Oryza sativa 
650 4 |a PROTEIN l-ISOASPARTYL O-METHYLTRANSFERASE (PIMT) 
650 4 |a aging 
650 4 |a isoaspartate 
650 4 |a longevity 
650 4 |a repair 
650 4 |a seed desiccation 
650 7 |a Isoenzymes  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a RNA, Messenger  |2 NLM 
650 7 |a Reactive Oxygen Species  |2 NLM 
650 7 |a Aspartic Acid  |2 NLM 
650 7 |a 30KYC7MIAI  |2 NLM 
650 7 |a Protein D-Aspartate-L-Isoaspartate Methyltransferase  |2 NLM 
650 7 |a EC 2.1.1.77  |2 NLM 
700 1 |a Kamble, Nitin Uttam  |e verfasserin  |4 aut 
700 1 |a Kumar, Meenu  |e verfasserin  |4 aut 
700 1 |a Verma, Pooja  |e verfasserin  |4 aut 
700 1 |a Ghosh, Shraboni  |e verfasserin  |4 aut 
700 1 |a Singh, Ajeet  |e verfasserin  |4 aut 
700 1 |a Rao, Venkateswara  |e verfasserin  |4 aut 
700 1 |a Salvi, Prafull  |e verfasserin  |4 aut 
700 1 |a Kaur, Harmeet  |e verfasserin  |4 aut 
700 1 |a Saxena, Saurabh Chandra  |e verfasserin  |4 aut 
700 1 |a Majee, Manoj  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t The New phytologist  |d 1979  |g 211(2016), 2 vom: 29. Juli, Seite 627-45  |w (DE-627)NLM09818248X  |x 1469-8137  |7 nnns 
773 1 8 |g volume:211  |g year:2016  |g number:2  |g day:29  |g month:07  |g pages:627-45 
856 4 0 |u http://dx.doi.org/10.1111/nph.13923  |3 Volltext 
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