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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1111/jpy.12285
|2 doi
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|a pubmed24n0861.xml
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|a eng
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| 100 |
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|a Chen, Jie
|e verfasserin
|4 aut
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| 245 |
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|a Transparent exopolymer particle production and aggregation by a marine planktonic diatom (Thalassiosira weissflogii) at different growth rates
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 26.07.2016
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|a Date Revised 18.03.2016
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a © 2015 Phycological Society of America.
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|a Transparent exopolymer particles (TEP) play an important role in the ocean carbon cycle as they are sticky and affect particle aggregation and the biological carbon pump. We investigated the effect of growth rate on TEP production in nitrogen limited semi-continuous cultures of the diatom Thalassiosira weissflogii (Grunow) G. Fryxell & Hasle. Steady-state diatom concentrations and other indicators of biomass (chl a, and total carbohydrate) were inversely related to growth rate, while individual cell volume increased with growth rate. There was no change in total TEP area with growth rate; however, individual TEP were larger at high growth rates and the number of individual TEP particles was lower. TEP concentration per cell was higher at higher growth rates. SYTOX Green staining showed that <5% of the diatom population had permeable cell membranes, with the proportion increasing at low growth rates. However, TEP production rates were greater at high growth rates, refuting our hypothesis that TEP formation is dependent on dying cells with compromised cell membranes in a diatom population. Measurements of particle size distribution in the cultures using laser scattering showed that they were most aggregated at high growth rates. These results indicate a coupling between TEP production and growth rate in diatoms under N limitation, with fast growing T. weissflogii producing more TEP and aggregates
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a SYTOX Green
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|a aggregation
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|a cell death
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|a cell volume
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|a diatom
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|a growth rate
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|a nitrogen limitation
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|a semi-continuous cultures
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|a transparent exopolymer particles
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|a Thornton, Daniel C O
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of phycology
|d 1966
|g 51(2015), 2 vom: 17. Apr., Seite 381-93
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|x 1529-8817
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|g volume:51
|g year:2015
|g number:2
|g day:17
|g month:04
|g pages:381-93
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|u http://dx.doi.org/10.1111/jpy.12285
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