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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.6b00553
|2 doi
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|a eng
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|a Akkarachaneeyakorn, Khrongkhwan
|e verfasserin
|4 aut
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|a Secretion and Reversible Assembly of Extracellular-like Matrix by Enzyme-Active Colloidosome-Based Protocells
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a Date Completed 13.03.2017
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|a Date Revised 13.03.2017
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The secretion and reversible assembly of an extracellular-like matrix by enzyme-active inorganic protocells (colloidosomes) is described. Addition of N-fluorenyl-methoxycarbonyl-tyrosine-(O)-phosphate to an aqueous suspension of alkaline phosphatase-containing colloidosomes results in molecular uptake and dephosphorylation to produce a time-dependent sequence of supramolecular hydrogel motifs (outer membrane wall, cytoskeletal-like interior and extra-protocellular matrix) that are integrated and remodelled within the microcapsule architecture and surrounding environment. Heat-induced disassembly of the extra-protocellular matrix followed by cooling produces colloidosomes with a densely packed hydrogel interior. These procedures are exploited for the fabrication of nested colloidosomes with spatially delineated regions of hydrogelation
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Colloids
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|a Fluorescent Dyes
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|a Hydrogels
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|a N-fluorenyl-methoxycarbonyl-tyrosine-(O)-phosphate
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|a Tyrosine
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|a Alkaline Phosphatase
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|a EC 3.1.3.1
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|a Li, Mei
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|a Davis, Sean A
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|a Mann, Stephen
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 32(2016), 12 vom: 29. März, Seite 2912-9
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|g year:2016
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|g month:03
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