Production of Transgenic Pigs with an Introduced Missense Mutation of the Bone Morphogenetic Protein Receptor Type IB Gene Related to Prolificacy

Production of Transgenic Pigs with an Introduced Missense Mutation of the Bone Morphogenetic Protein Receptor Type IB Gene Related to Prolificacy

In the last few decades, transgenic animal technology has witnessed an increasingly wide application in animal breeding. Reproductive traits are economically important to the pig industry. It has been shown that the bone morphogenetic protein receptor type IB (BMPR1B) A746G polymorphism is responsib...

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Veröffentlicht in:Asian-Australasian journal of animal sciences. - 1998. - 29(2016), 7 vom: 18. Juli, Seite 925-37
1. Verfasser: Zhao, Xueyan (VerfasserIn)
Weitere Verfasser: Yang, Qiang, Zhao, Kewei, Jiang, Chao, Ren, Dongren, Xu, Pan, He, Xiaofang, Liao, Rongrong, Jiang, Kai, Ma, Junwu, Xiao, Shijun, Ren, Jun, Xing, Yuyun
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Asian-Australasian journal of animal sciences
Schlagworte:Journal Article BMPR1B Coding Sequence Handmade Cloning Pig Reproductive Traits Transgenic
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520 |a In the last few decades, transgenic animal technology has witnessed an increasingly wide application in animal breeding. Reproductive traits are economically important to the pig industry. It has been shown that the bone morphogenetic protein receptor type IB (BMPR1B) A746G polymorphism is responsible for the fertility in sheep. However, this causal mutation exits exclusively in sheep and goat. In this study, we attempted to create transgenic pigs by introducing this mutation with the aim to improve reproductive traits in pigs. We successfully constructed a vector containing porcine BMPR1B coding sequence (CDS) with the mutant G allele of A746G mutation. In total, we obtained 24 cloned male piglets using handmade cloning (HMC) technique, and 12 individuals survived till maturation. A set of polymerase chain reactions indicated that 11 of 12 matured boars were transgene-positive individuals, and that the transgenic vector was most likely disrupted during cloning. Of 11 positive pigs, one (No. 11) lost a part of the terminator region but had the intact promoter and the CDS regions. cDNA sequencing showed that the introduced allele (746G) was expressed in multiple tissues of transgene-positive offspring of No.11. Western blot analysis revealed that BMPR1B protein expression in multiple tissues of transgene-positive F1 piglets was 0.5 to 2-fold higher than that in the transgene-negative siblings. The No. 11 boar showed normal litter size performance as normal pigs from the same breed. Transgene-positive F1 boars produced by No. 11 had higher semen volume, sperm concentration and total sperm per ejaculate than the negative siblings, although the differences did not reached statistical significance. Transgene-positive F1 sows had similar litter size performance to the negative siblings, and more data are needed to adequately assess the litter size performance. In conclusion, we obtained 24 cloned transgenic pigs with the modified porcine BMPR1B CDS using HMC. cDNA sequencing and western blot indicated that the exogenous BMPR1B CDS was successfully expressed in host pigs. The transgenic pigs showed normal litter size performance. However, no significant differences in litter size were found between transgene-positive and negative sows. Our study provides new insight into producing cloned transgenic livestock related to reproductive traits 
650 4 |a Journal Article 
650 4 |a BMPR1B 
650 4 |a Coding Sequence 
650 4 |a Handmade Cloning 
650 4 |a Pig 
650 4 |a Reproductive Traits 
650 4 |a Transgenic 
700 1 |a Yang, Qiang  |e verfasserin  |4 aut 
700 1 |a Zhao, Kewei  |e verfasserin  |4 aut 
700 1 |a Jiang, Chao  |e verfasserin  |4 aut 
700 1 |a Ren, Dongren  |e verfasserin  |4 aut 
700 1 |a Xu, Pan  |e verfasserin  |4 aut 
700 1 |a He, Xiaofang  |e verfasserin  |4 aut 
700 1 |a Liao, Rongrong  |e verfasserin  |4 aut 
700 1 |a Jiang, Kai  |e verfasserin  |4 aut 
700 1 |a Ma, Junwu  |e verfasserin  |4 aut 
700 1 |a Xiao, Shijun  |e verfasserin  |4 aut 
700 1 |a Ren, Jun  |e verfasserin  |4 aut 
700 1 |a Xing, Yuyun  |e verfasserin  |4 aut 
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773 1 8 |g volume:29  |g year:2016  |g number:7  |g day:18  |g month:07  |g pages:925-37 
856 4 0 |u http://dx.doi.org/10.5713/ajas.15.0505  |3 Volltext 
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