Overexpression of Rosa rugosa anthocyanidin reductase enhances tobacco tolerance to abiotic stress through increased ROS scavenging and modulation of ABA signaling
Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.
| Veröffentlicht in: | Plant science : an international journal of experimental plant biology. - 1985. - 245(2016) vom: 28. Apr., Seite 35-49 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2016
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| Zugriff auf das übergeordnete Werk: | Plant science : an international journal of experimental plant biology |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't ABA ABF ANR Proanthocyanidins ROS Stress tolerance Antioxidants Flavonoids mehr... |
| Zusammenfassung: | Copyright © 2016 Elsevier Ireland Ltd. All rights reserved. Anthocyanidin reductase (ANR) is a key enzyme involved in the biosynthesis of proanthocyanidins (PAs) and plays a role in the plant stress response. However, the mechanism by which ANR confers stress tolerance in plants is not understood. Here, we report the isolation of RrANR, the homologous gene from rose, and NtABF, an ABA-response related transcription factor gene from tobacco. These genes were characterized regarding their functions in stress responses through the use of transgenic, transcriptomic and physiological analyses. Over-expression of RrANR in tobacco resulted in an increased accumulation of both PAs and abscisic acid (ABA), and also enhanced stress tolerance. Transcriptomic analysis of these transgenic tobacco lines indicated that RrANR overexpression induced global transcriptomic changes, including these involved in oxidation/reduction, hormone response and secondary metabolism. Genes related to ABA biosynthesis and reactive oxygen species (ROS)-scavenging were up-regulated in RrANR transgenic lines, and these effects were phenocopied by the direct treatment of tobacco plants with PAs and ABA. Transcriptomic data from each of these treatments identified the upregulation of a putative NtABF. Furthermore, the up-regulation of NtABF in RrANR transformants or in PAs- and ABA-treated tobacco plants was associated with enhanced stress tolerance. Overexpression of NtABF in transgenic tobacco mimicked the effects of RrANR-transgenic plants with regard to the up-regulation of ROS-scavenging genes and an increase in oxidative tolerance. Taken together, our findings indicate that overexpression of RrANR results in an increase in plant tolerance to oxidative stress via increased scavenging of ROS and modulation of the ABA signaling pathway |
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| Beschreibung: | Date Completed 13.12.2016 Date Revised 13.12.2023 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2259 |
| DOI: | 10.1016/j.plantsci.2016.01.007 |