Pepper CabZIP63 acts as a positive regulator during Ralstonia solanacearum or high temperature-high humidity challenge in a positive feedback loop with CaWRKY40

Pepper CabZIP63 acts as a positive regulator during Ralstonia solanacearum or high temperature-high humidity challenge in a positive feedback loop with CaWRKY40

© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 67(2016), 8 vom: 15. Apr., Seite 2439-51
1. Verfasser: Shen, Lei (VerfasserIn)
Weitere Verfasser: Liu, Zhiqin, Yang, Sheng, Yang, Tong, Liang, Jiaqi, Wen, Jiayu, Liu, Yanyan, Li, Jiazhi, Shi, Lanping, Tang, Qian, Shi, Wei, Hu, Jiong, Liu, Cailing, Zhang, Yangwen, Lin, Wei, Wang, Rongzhang, Yu, Huanxin, Mou, Shaoliang, Hussain, Ansar, Cheng, Wei, Cai, Hanyang, He, Li, Guan, Deyi, Wu, Yang, He, Shuilin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't CaWRKY40 CabZIP63 Ralstonia solanacearum high temperature–high humidity pepper transcription factors. Acetates Biomarkers mehr... Cyclopentanes Ethylenes Oxylipins Plant Proteins Abscisic Acid 72S9A8J5GW methyl jasmonate 900N171A0F
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500 |a Date Revised 13.11.2018 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. 
520 |a CaWRKY40 is known to act as a positive regulator in the response of pepper (Capsicum annuum) to Ralstonia solanacearum inoculation (RSI) or high temperature-high humidity (HTHH), but the underlying mechanism remains elusive. Herein, we report that CabZIP63, a pepper bZIP family member, participates in this process by regulating the expression of CaWRKY40. CabZIP63 was found to localize in the nuclei, be up-regulated by RSI or HTHH, bind to promoters of both CabZIP63(pCabZIP63) and CaWRKY40(pCaWRKY40), and activate pCabZIP63- and pCaWRKY40-driven β-glucuronidase expression in a C- or G-box-dependent manner. Silencing of CabZIP63 by virus-induced gene silencing (VIGS) in pepper plants significantly attenuated their resistance to RSI and tolerance to HTHH, accompanied by down-regulation of immunity- or thermotolerance-associated CaPR1, CaNPR1, CaDEF1, and CaHSP24. Hypersensitive response-mediated cell death and expression of the tested immunity- and thermotolerance-associated marker genes were induced by transient overexpression (TOE) of CabZIP63, but decreased by that of CabZIP63-SRDX. Additionally, binding of CabZIP63 to pCaWRKY40 was up-regulated by RSI or HTHH, and the transcript level of CaWRKY40 and binding of CaWRKY40 to the promoters of CaPR1, CaNPR1, CaDEF1 and CaHSP24 were up-regulated by TOE of CabZIP63. On the other hand, CabZIP63 was also up-regulated transcriptionally by TOE of CaWRKY40. The data suggest collectively that CabZIP63 directly or indirectly regulates the expression of CaWRKY40 at both the transcriptional and post-transcriptional level, forming a positive feedback loop with CaWRKY40 during pepper's response to RSI or HTHH. Altogether, our data will help to elucidate the underlying mechanism of crosstalk between pepper's response to RSI and HTHH 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a CaWRKY40 
650 4 |a CabZIP63 
650 4 |a Ralstonia solanacearum 
650 4 |a high temperature–high humidity 
650 4 |a pepper 
650 4 |a transcription factors. 
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650 7 |a Biomarkers  |2 NLM 
650 7 |a Cyclopentanes  |2 NLM 
650 7 |a Ethylenes  |2 NLM 
650 7 |a Oxylipins  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Abscisic Acid  |2 NLM 
650 7 |a 72S9A8J5GW  |2 NLM 
650 7 |a methyl jasmonate  |2 NLM 
650 7 |a 900N171A0F  |2 NLM 
700 1 |a Liu, Zhiqin  |e verfasserin  |4 aut 
700 1 |a Yang, Sheng  |e verfasserin  |4 aut 
700 1 |a Yang, Tong  |e verfasserin  |4 aut 
700 1 |a Liang, Jiaqi  |e verfasserin  |4 aut 
700 1 |a Wen, Jiayu  |e verfasserin  |4 aut 
700 1 |a Liu, Yanyan  |e verfasserin  |4 aut 
700 1 |a Li, Jiazhi  |e verfasserin  |4 aut 
700 1 |a Shi, Lanping  |e verfasserin  |4 aut 
700 1 |a Tang, Qian  |e verfasserin  |4 aut 
700 1 |a Shi, Wei  |e verfasserin  |4 aut 
700 1 |a Hu, Jiong  |e verfasserin  |4 aut 
700 1 |a Liu, Cailing  |e verfasserin  |4 aut 
700 1 |a Zhang, Yangwen  |e verfasserin  |4 aut 
700 1 |a Lin, Wei  |e verfasserin  |4 aut 
700 1 |a Wang, Rongzhang  |e verfasserin  |4 aut 
700 1 |a Yu, Huanxin  |e verfasserin  |4 aut 
700 1 |a Mou, Shaoliang  |e verfasserin  |4 aut 
700 1 |a Hussain, Ansar  |e verfasserin  |4 aut 
700 1 |a Cheng, Wei  |e verfasserin  |4 aut 
700 1 |a Cai, Hanyang  |e verfasserin  |4 aut 
700 1 |a He, Li  |e verfasserin  |4 aut 
700 1 |a Guan, Deyi  |e verfasserin  |4 aut 
700 1 |a Wu, Yang  |e verfasserin  |4 aut 
700 1 |a He, Shuilin  |e verfasserin  |4 aut 
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773 1 8 |g volume:67  |g year:2016  |g number:8  |g day:15  |g month:04  |g pages:2439-51 
856 4 0 |u http://dx.doi.org/10.1093/jxb/erw069  |3 Volltext 
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