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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2016.02.001
|2 doi
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|a pubmed25n0858.xml
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|a (DE-627)NLM257652922
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|a (NLM)26895330
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|a (PII)S0176-1617(16)00018-3
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Ranganathan, Kapilan
|e verfasserin
|4 aut
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|a Responses of hybrid aspen over-expressing a PIP2;5 aquaporin to low root temperature
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 28.08.2017
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2016 Elsevier GmbH. All rights reserved.
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|a Aquaporins mediate the movement of water across cell membranes. Plasma membrane intrinsic protein 2;5 from Populus trichocarpa×deltoides (PtdPIP2;5) was previously demonstrated to be a functionally important water conducting aquaporin. To study the relevance of aquaporin-mediated root water transport at low temperatures, we generated transgenic Populus tremula×alba over-expressing PtdPIP2;5 under control of the maize ubiquitin promoter, and compared the physiological responses and water transport properties of the PtdPIP2;5 over-expressing lines (PtdPIP2;5ox) with wild-type plants. We hypothesized that over-expression of PtdPIP2;5 would reduce temperature sensitivity of root water transport and gas exchange. Decreasing root temperatures to 10 and 5°C significantly decreased hydraulic conductivities (Lp) in wild-type plants, but had no significant effect on Lp in PtdPIP2;5ox plants. Recovery of Lp in the transgenic lines returned to 20°C from 5°C was faster than in the wild-type plants. Low root temperature did not induce major changes in transcript levels for other PIPs. When roots were exposed to 5°C in solution culture and shoots were exposed to 20°C, wild-type plants had significantly lower net photosynthetic and transpiration rates compared to PtdPIP2;5ox plants. Taken together, our results demonstrate that over-expression of PtdPIP2;5 in P. tremula×alba was effective in alleviating the effects of low root temperature on Lp and gas exchange
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Aquaporin
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|a Hydraulic conductivity
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|a Low root temperature
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|a PIP2;5
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|a Water transport
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|a Aquaporins
|2 NLM
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Ubiquitin
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|a Water
|2 NLM
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|a 059QF0KO0R
|2 NLM
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|a El Kayal, Walid
|e verfasserin
|4 aut
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|a Cooke, Janice E K
|e verfasserin
|4 aut
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|a Zwiazek, Janusz J
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 192(2016) vom: 15. März, Seite 98-104
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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|g volume:192
|g year:2016
|g day:15
|g month:03
|g pages:98-104
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|u http://dx.doi.org/10.1016/j.jplph.2016.02.001
|3 Volltext
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