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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erw019
|2 doi
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|a pubmed24n1344.xml
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|a (DE-627)NLM257591389
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|a (NLM)26889010
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Xu, Yi
|e verfasserin
|4 aut
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|a Enhancing cytokinin synthesis by overexpressing ipt alleviated drought inhibition of root growth through activating ROS-scavenging systems in Agrostis stolonifera
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 13.12.2016
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|a Date Revised 24.03.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Drought stress limits root growth and inhibits cytokinin (CK) production. Increases in CK production through overexpression of isopentenyltransferase (ipt) alleviate drought damages to promote root growth. The objective of this study was to investigate whether CK-regulated root growth was involved in the alteration of reactive oxygen species (ROS) production and ROS scavenging capacity under drought stress. Wild-type (WT) creeping bentgrass (Agrostis stolonifera L. 'Penncross') and a transgenic line (S41) overexpressing ipt ligated to a senescence-activated promoter (SAG12) were exposed to drought stress for 21 d in growth chambers. SAG12-ipt transgenic S41 developed a more extensive root system under drought stress compared to the WT. Root physiological analysis (electrolyte leakage and lipid peroxidation) showed that S41 roots exhibited less cellular damage compared to the WT under drought stress. Roots of SAG12-ipt transgenic S41 had significantly higher endogenous CK content than the WT roots under drought stress. ROS (hydrogen peroxide and superoxide) content was significantly lower and content of total and free ascorbate was significantly higher in S41 roots compared to the WT roots under drought stress. Enzymatic assays and transcript abundance analysis showed that superoxide dismutase, catalase, peroxidase, and dehydroascorbate reductase were significantly higher in S41 roots compared to the WT roots under drought stress. S41 roots also maintained significantly higher alternative respiration rates compared to the WT under drought stress. The improved root growth of transgenic creeping bentgrass may be facilitated by CK-enhanced ROS scavenging through antioxidant accumulation and activation of antioxidant enzymes, as well as higher alternative respiration rates when soil water is limited
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Antioxidant
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|a ROS scavenging system
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|a drought stress
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|a qRT-PCR
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|a root respiration
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|a turfgrass.
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|a Antioxidants
|2 NLM
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|a Cytokinins
|2 NLM
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|a Electrolytes
|2 NLM
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|a Free Radical Scavengers
|2 NLM
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|a RNA, Messenger
|2 NLM
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|a Reactive Oxygen Species
|2 NLM
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|a Superoxides
|2 NLM
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|a 11062-77-4
|2 NLM
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|a Nitroprusside
|2 NLM
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|a 169D1260KM
|2 NLM
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|a Malondialdehyde
|2 NLM
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|a 4Y8F71G49Q
|2 NLM
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|a Catalase
|2 NLM
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|a EC 1.11.1.6
|2 NLM
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|a Superoxide Dismutase
|2 NLM
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| 650 |
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|a EC 1.15.1.1
|2 NLM
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|a Alkyl and Aryl Transferases
|2 NLM
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| 650 |
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|a EC 2.5.-
|2 NLM
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|a adenylate isopentenyltransferase
|2 NLM
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|a EC 2.5.1.27
|2 NLM
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|a Glutathione
|2 NLM
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|a GAN16C9B8O
|2 NLM
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|a Ascorbic Acid
|2 NLM
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| 650 |
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|a PQ6CK8PD0R
|2 NLM
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| 700 |
1 |
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|a Burgess, Patrick
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Xunzhong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Huang, Bingru
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 67(2016), 6 vom: 18. März, Seite 1979-92
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:67
|g year:2016
|g number:6
|g day:18
|g month:03
|g pages:1979-92
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erw019
|3 Volltext
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|d 67
|j 2016
|e 6
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|h 1979-92
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