|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM257548327 |
| 003 |
DE-627 |
| 005 |
20231227125330.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2016 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/erw014
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1223.xml
|
| 035 |
|
|
|a (DE-627)NLM257548327
|
| 035 |
|
|
|a (NLM)26884602
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Liu, Yuhui
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Functional diversification of the potato R2R3 MYB anthocyanin activators AN1, MYBA1, and MYB113 and their interaction with basic helix-loop-helix cofactors
|
| 264 |
|
1 |
|c 2016
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 13.12.2016
|
| 500 |
|
|
|a Date Revised 13.12.2023
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.
|
| 520 |
|
|
|a In potato (Solanum tuberosum L.), R2R3 MYBs are involved in the regulation of anthocyanin biosynthesis. We examined sequences of these MYBs in cultivated potatoes, which are more complex than diploid potato due to ploidy and heterozygosity. We found amino acid variants in the C-terminus of the MYB StAN1, termed R0, R1, and R3, due to the presence of a repeated 10-amino acid motif. These variant MYBs showed some expression in both white and pigmented tubers. We found several new alleles or gene family members of R2R3 MYBs,StMYBA1 and StMYB113, which were also expressed in white potato tubers. From functional analysis in tobacco, we showed that the presence of a C-terminal 10-amino acid motif is optimal for activating anthocyanin accumulation. Engineering a motif back into a MYB lacking this sequence enhanced its activating ability. Versions of StMYBA1 and StMYB113 can also activate anthocyanin accumulation in tobacco leaves, with the exception of StMYB113-3, which has a partial R2R3 domain. We isolated five family members of potato StbHLH1, and one StJAF13, to test their ability to interact with MYB variants. The results showed that two alleles of StbHLH1 from white skin and red skin are non-functional, while three other StbHLH1s have different co-regulating abilities, and need to be activated by StJAF13. Combined with expression analysis in potato tuber, results suggest that StbHLH1 and StJAF13a re key co-regulators of anthocyanin biosynthesis, while the transcripts of MYB variants StAN1,StMYBA1, and StMYB113 are well expressed, even in the absence of pigmentation
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Anthocyanin
|
| 650 |
|
4 |
|a MYB transcription factors
|
| 650 |
|
4 |
|a cofactors
|
| 650 |
|
4 |
|a diversification
|
| 650 |
|
4 |
|a interaction
|
| 650 |
|
4 |
|a potato.
|
| 650 |
|
7 |
|a Anthocyanins
|2 NLM
|
| 650 |
|
7 |
|a Basic Helix-Loop-Helix Transcription Factors
|2 NLM
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a RNA, Messenger
|2 NLM
|
| 700 |
1 |
|
|a Lin-Wang, Kui
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Espley, Richard V
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wang, Li
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Yang, Hongyu
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Yu, Bin
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Dare, Andrew
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Varkonyi-Gasic, Erika
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wang, Jing
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zhang, Junlian
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wang, Di
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Allan, Andrew C
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 67(2016), 8 vom: 17. Apr., Seite 2159-76
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:67
|g year:2016
|g number:8
|g day:17
|g month:04
|g pages:2159-76
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/erw014
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 67
|j 2016
|e 8
|b 17
|c 04
|h 2159-76
|