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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erw018
|2 doi
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|a pubmed24n0857.xml
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|a (DE-627)NLM257149309
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|a (NLM)26842983
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Siena, Lorena A
|e verfasserin
|4 aut
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| 245 |
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|a An apomixis-linked ORC3-like pseudogene is associated with silencing of its functional homolog in apomictic Paspalum simplex
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 13.12.2016
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|a Date Revised 30.12.2016
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Apomixis in plants consists of asexual reproduction by seeds. Here we characterized at structural and functional levels an apomixis-linked sequence of Paspalum simplex homologous to subunit 3 of the ORIGIN RECOGNITION COMPLEX (ORC3). ORC is a multiprotein complex which controls DNA replication and cell differentiation in eukaryotes. Three PsORC3 copies were identified, each one characterized by a specific expression profile. Of these, PsORC3a, specific for apomictic genotypes, is a pseudogene that was poorly and constitutively expressed in all developmental stages of apomictic flowers, whereas PsORC3b, the putative functional gene in sexual flowers, showed a precise time-related regulation. Sense transcripts of PsORC3 were expressed in the female cell lineage of both apomictic and sexual reproductive phenotypes, and in aposporous initials. Although strong expression was detected in sexual early endosperm, no expression was present in the apomictic endosperm. Antisense PsORC3 transcripts were revealed exclusively in apomictic germ cell lineages. Defective orc3 mutants of rice and Arabidopsis showed normal female gametophytes although the embryo and endosperm were arrested at early phases of development. We hypothesize that PsORC3a is associated with the down-regulation of its functional homolog and with the development of apomictic endosperm which deviates from the canonical 2(maternal):1(paternal) genome ratio
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Apomixis
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| 650 |
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4 |
|a Paspalum
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| 650 |
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|a gene silencing
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| 650 |
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|a origin recognition complex
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|a seed development.
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a RNA, Messenger
|2 NLM
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| 700 |
1 |
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|a Ortiz, Juan Pablo A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Calderini, Ornella
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Paolocci, Francesco
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cáceres, Maria E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kaushal, Pankaj
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Grisan, Simone
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pessino, Silvina C
|e verfasserin
|4 aut
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| 700 |
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|a Pupilli, Fulvio
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 67(2016), 6 vom: 14. März, Seite 1965-78
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:67
|g year:2016
|g number:6
|g day:14
|g month:03
|g pages:1965-78
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erw018
|3 Volltext
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|d 67
|j 2016
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|h 1965-78
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