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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1111/vcp.12324
|2 doi
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|a eng
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| 100 |
1 |
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|a Williams, Tim L
|e verfasserin
|4 aut
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|a Validation of an automated enzyme immunoassay for the measurement of serum total thyroxine in cats
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|c 2016
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|a Text
|b txt
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|a ƒaComputermedien
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|a Date Completed 28.02.2018
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|a Date Revised 10.12.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2016 American Society for Veterinary Clinical Pathology.
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|a BACKGROUND: Hyperthyroidism is common in older cats, which necessitates frequent screening of serum total thyroxine (TT4) concentrations. Fast, cheap, and reliable methods to measure TT4 in cats are needed
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|a OBJECTIVES: The purpose of the study was the validation of a human TT4 enzyme immunoassay (EIA) for use with feline serum, and derivation of a TT4 reference interval (RI) for cats aged 9 years and older
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|a METHODS: Assay precision, reproducibility, and linearity were evaluated. Interference by hemolysis was also assessed. Method comparison studies between the human EIA and a previously validated radioimmunoassay (RIA) and chemiluminescent-enzyme immunoassay (CEIA) were performed. Healthy cats (> 9 years) were recruited from 3 UK first opinion practices
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|a RESULTS: The human TT4 EIA demonstrated good precision and reproducibility, and adequate linearity. Hemolysis did not significantly alter measured TT4 concentrations until HGB > 8 g/L. Method comparison revealed proportional and constant errors between EIA and RIA/CEIA. The TT4 RI for cats (> 9 years) was calculated as 7.1-45.1 nmol/L (n = 49)
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|a CONCLUSIONS: The human TT4 EIA was successfully validated for use with feline serum and offers a rapid, cheap, and reliable method for determination of serum TT4 concentrations in cats
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|a Journal Article
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|a Validation Study
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|a Analyzer
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|a feline
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|a human
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|a reference interval
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|a Archer, Joy
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|t Veterinary clinical pathology
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|g 45(2016), 1 vom: 01. März, Seite 148-53
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|g volume:45
|g year:2016
|g number:1
|g day:01
|g month:03
|g pages:148-53
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|u http://dx.doi.org/10.1111/vcp.12324
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