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231224s2016 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1111/vcp.12327
|2 doi
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5 |
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|a pubmed24n0855.xml
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|a (DE-627)NLM256762945
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|a (NLM)26802284
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Eschbaumer, Michael
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Effect of storage conditions on subpopulations of peripheral blood T lymphocytes isolated from naïve cattle and cattle infected with foot-and-mouth disease virus
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| 264 |
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1 |
|c 2016
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 28.02.2018
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| 500 |
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|a Date Revised 28.02.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a © 2016 American Society for Veterinary Clinical Pathology.
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|a BACKGROUND: Immunophenotyping of blood lymphocytes by flow cytometry is important in infectious disease research. In animal experiments and other longitudinal studies, the processing, prompt staining, and analysis of fresh samples is a logistical challenge and daily assay variation can confound data interpretation
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|a OBJECTIVE: This study examined the feasibility of cryopreservation and deferred analysis of bovine peripheral blood T lymphocytes from normal or infected animals
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|a METHODS: Peripheral blood mononuclear cells were collected from 4 naïve Holstein steers and 4 steers infected with foot-and-mouth-disease virus serotype Asia1. Identical aliquots were labeled and analyzed immediately, labeled for deferred analysis, or stored at -70°C or over liquid nitrogen for up to 3 weeks before labeling
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| 520 |
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|a RESULTS: Freezing of unlabeled cells induced statistically significant changes in phenotypic recognition. In infected animals, the γδ T-cell population increased by 28% and CD8(+) αβT cells by 32%, while total CD3(+) cells decreased by 16%, and CD4(+) αβT cells decreased by 12%. Subsequent storage of frozen cells for the duration of the study, however, had no significant effect. There was less than 20% relative change in subpopulation sizes, and storage at -70°C or over liquid nitrogen was equivalent
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| 520 |
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|a CONCLUSIONS: Depending on the objectives and practical limitations of a study, deferred labeling of peripheral blood lymphocytes can be a viable option. Although frozen storage of lymphocytes can introduce some artifactual distortion of relative cell populations, frozen cells can be maintained in storage until all samples in a longitudinal study can be analyzed in batch under standardized conditions and without introducing further bias
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Cryopreservation
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| 650 |
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4 |
|a flow cytometry
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| 650 |
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4 |
|a immunophenotyping
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| 650 |
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4 |
|a peripheral blood mononuclear cells
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| 650 |
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4 |
|a storage
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| 700 |
1 |
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|a Stenfeldt, Carolina
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pacheco, Juan M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rekant, Steven I
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Arzt, Jonathan
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 45(2016), 1 vom: 14. März, Seite 110-5
|w (DE-627)NLM098159984
|x 1939-165X
|7 nnns
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| 773 |
1 |
8 |
|g volume:45
|g year:2016
|g number:1
|g day:14
|g month:03
|g pages:110-5
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| 856 |
4 |
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|u http://dx.doi.org/10.1111/vcp.12327
|3 Volltext
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