Increased abscisic acid levels in transgenic maize overexpressing AtLOS5 mediated root ion fluxes and leaf water status under salt stress

Increased abscisic acid levels in transgenic maize overexpressing AtLOS5 mediated root ion fluxes and leaf water status under salt stress

© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 67(2016), 5 vom: 18. März, Seite 1339-55
1. Verfasser: Zhang, Juan (VerfasserIn)
Weitere Verfasser: Yu, Haiyue, Zhang, Yushi, Wang, Yubing, Li, Maoying, Zhang, Jiachang, Duan, Liusheng, Zhang, Mingcai, Li, Zhaohu
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't ABA AtLOS5 ion fluxes maize salt stress water uptake. Arabidopsis Proteins Ions mehr... Water 059QF0KO0R Mannitol 3OWL53L36A Vanadates 3WHH0066W5 Sodium Chloride 451W47IQ8X Abscisic Acid 72S9A8J5GW Amiloride 7DZO8EB0Z3 Hydrogen 7YNJ3PO35Z Sodium 9NEZ333N27 Aldehyde Oxidase EC 1.2.3.1 ABA3 protein, Arabidopsis EC 2.8.1.- Sulfurtransferases Potassium RWP5GA015D
LEADER 01000caa a22002652 4500
001 NLM256249644
003 DE-627
005 20250219132502.0
007 cr uuu---uuuuu
008 231224s2016 xx |||||o 00| ||eng c
024 7 |a 10.1093/jxb/erv528  |2 doi 
028 5 2 |a pubmed25n0854.xml 
035 |a (DE-627)NLM256249644 
035 |a (NLM)26743432 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Zhang, Juan  |e verfasserin  |4 aut 
245 1 0 |a Increased abscisic acid levels in transgenic maize overexpressing AtLOS5 mediated root ion fluxes and leaf water status under salt stress 
264 1 |c 2016 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 13.12.2016 
500 |a Date Revised 16.03.2022 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. 
520 |a Abscisic acid (ABA) is a vital cellular signal in plants, and effective ABA signalling is pivotal for stress tolerance. AtLOS5 encoding molybdenum cofactor sulphurase is a key regulator of ABA biosynthesis. Here, transgenic AtLOS5 plants were generated to explore the role of AtLOS5 in salt tolerance in maize. AtLOS5 overexpression significantly up-regulated the expression of ZmVp14-2, ZmAO, and ZmMOCO, and increased aldehyde oxidase activities, which enhanced ABA accumulation in transgenic plants under salt stress. Concurrently, AtLOS5 overexpression induced the expression of ZmNHX1, ZmCBL4, and ZmCIPK16, and enhanced the root net Na(+) efflux and H(+) influx, but decreased net K(+) efflux, which maintained a high cytosolic K(+)/Na(+) ratio in transgenic plants under salt stress. However, amiloride or sodium orthovanadate could significantly elevate K(+) effluxes and decrease Na(+) efflux and H(+) influx in salt-treated transgenic roots, but the K(+) effluxes were inhibited by TEA, suggesting that ion fluxes regulated by AtLOS5 overexpression were possibly due to activation of Na(+)/H(+) antiport and K(+) channels across the plasma membrane. Moreover, AtLOS5 overexpression could up-regulate the transcripts of ZmPIP1:1, ZmPIP1:5, and ZmPIP2:4, and enhance root hydraulic conductivity. Thus transgenic plants had higher leaf water potential and turgor, which was correlated with greater biomass accumulation under salt stress. Thus AtLOS5 overexpression induced the expression of ABA biosynthetic genes to promote ABA accumulation, which activated ion transporter and PIP aquaporin gene expression to regulate root ion fluxes and water uptake, thus maintaining high cytosolic K(+) and Na(+) homeostasis and better water status in maize exposed to salt stress 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a ABA 
650 4 |a AtLOS5 
650 4 |a ion fluxes 
650 4 |a maize 
650 4 |a salt stress 
650 4 |a water uptake. 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a Ions  |2 NLM 
650 7 |a Water  |2 NLM 
650 7 |a 059QF0KO0R  |2 NLM 
650 7 |a Mannitol  |2 NLM 
650 7 |a 3OWL53L36A  |2 NLM 
650 7 |a Vanadates  |2 NLM 
650 7 |a 3WHH0066W5  |2 NLM 
650 7 |a Sodium Chloride  |2 NLM 
650 7 |a 451W47IQ8X  |2 NLM 
650 7 |a Abscisic Acid  |2 NLM 
650 7 |a 72S9A8J5GW  |2 NLM 
650 7 |a Amiloride  |2 NLM 
650 7 |a 7DZO8EB0Z3  |2 NLM 
650 7 |a Hydrogen  |2 NLM 
650 7 |a 7YNJ3PO35Z  |2 NLM 
650 7 |a Sodium  |2 NLM 
650 7 |a 9NEZ333N27  |2 NLM 
650 7 |a Aldehyde Oxidase  |2 NLM 
650 7 |a EC 1.2.3.1  |2 NLM 
650 7 |a ABA3 protein, Arabidopsis  |2 NLM 
650 7 |a EC 2.8.1.-  |2 NLM 
650 7 |a Sulfurtransferases  |2 NLM 
650 7 |a EC 2.8.1.-  |2 NLM 
650 7 |a Potassium  |2 NLM 
650 7 |a RWP5GA015D  |2 NLM 
700 1 |a Yu, Haiyue  |e verfasserin  |4 aut 
700 1 |a Zhang, Yushi  |e verfasserin  |4 aut 
700 1 |a Wang, Yubing  |e verfasserin  |4 aut 
700 1 |a Li, Maoying  |e verfasserin  |4 aut 
700 1 |a Zhang, Jiachang  |e verfasserin  |4 aut 
700 1 |a Duan, Liusheng  |e verfasserin  |4 aut 
700 1 |a Zhang, Mingcai  |e verfasserin  |4 aut 
700 1 |a Li, Zhaohu  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of experimental botany  |d 1985  |g 67(2016), 5 vom: 18. März, Seite 1339-55  |w (DE-627)NLM098182706  |x 1460-2431  |7 nnns 
773 1 8 |g volume:67  |g year:2016  |g number:5  |g day:18  |g month:03  |g pages:1339-55 
856 4 0 |u http://dx.doi.org/10.1093/jxb/erv528  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 67  |j 2016  |e 5  |b 18  |c 03  |h 1339-55