Establishing a herbicide-metabolizing enzyme library in Beckmannia syzigachne to identify genes associated with metabolic resistance

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 67(2016), 6 vom: 07. März, Seite 1745-57
1. Verfasser:	Pan, Lang (VerfasserIn)
Weitere Verfasser:	Gao, Haitao, Xia, Wenwen, Zhang, Teng, Dong, Liyao
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2016
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Abiotic stress Beckmannia syzigachne fenoxaprop-P-ethyl-resistant population metabolic resistance metabolizing enzyme library molecular mechanisms. Amino Acids Herbicides mehr... Oxazoles Propionates fenoxaprop ethyl 7U20WEM458

Beschreibung
Zusammenfassung:	© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. Non-target site resistance (NTSR) to herbicides is an increasing concern for weed control. Metabolic herbicide resistance is an important mechanism for NTSR. However, little is known about metabolic resistance at the genetic level. In this study, we have identified three fenoxaprop-P-ethyl-resistant American sloughgrass (Beckmannia syzigachne Steud.) populations, in which the molecular basis for NTSR remains unclear. To reveal the mechanisms of metabolic resistance, the genes likely to be involved in herbicide metabolism (e.g. for cytochrome P450s, esterases, hydrolases, oxidases, peroxidases, glutathione S-transferases, glycosyltransferases, and transporter proteins) were isolated using transcriptome sequencing, in combination with RT-PCR (reverse transcription-PCR) and RACE (rapid amplification of cDNA ends). Consequently, we established a herbicide-metabolizing enzyme library containing at least 332 genes, and each of these genes was cloned and the sequence and the expression level compared between the fenoxaprop-P-ethyl-resistant and susceptible populations. Fifteen metabolic enzyme genes were found to be possibly involved in fenoxaprop-P-ethyl resistance. In addition, we found five metabolizing enzyme genes that have a different gene sequence in plants of susceptible versus resistant B. syzigachne populations. These genes may be major candidates for herbicide metabolic resistance. This established metabolic enzyme library represents an important step forward towards a better understanding of herbicide metabolism and metabolic resistance in this and possibly other closely related weed species. This new information may help to understand weed metabolic resistance and to develop novel strategies of weed management
Beschreibung:	Date Completed 13.12.2016 Date Revised 16.11.2017 published: Print-Electronic Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/erv565