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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2015.12.008
|2 doi
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|a pubmed24n0853.xml
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|a (NLM)26730883
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|a (PII)S0981-9428(15)30187-X
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Kanwal, Simab
|e verfasserin
|4 aut
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| 245 |
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|a Characterization of glutamate decarboxylase from Synechocystis sp. PCC6803 and its role in nitrogen metabolism
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 20.10.2016
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2015 Elsevier Masson SAS. All rights reserved.
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|a Glutamate decarboxylase (GAD) (EC 4.1.1.15), an enzyme responsible for the synthesis of γ-aminobutyric acid (GABA), from Synechocystis sp. PCC6803 was cloned and overexpressed in Escherichia coli BL21(DE3). The purified enzyme was expressed as a monomeric protein with a molecular mass of 53 and 55 kDa as determined by SDS-PAGE and gel filtration chromatography, respectively. The enzyme activity was pyridoxal-5'-phosphate dependent with an optimal activity at pH 6.0 and 30 °C. The catalytic properties of this enzyme were, Km = 19.6 mM; kcat = 100.7 s(-1); and kcat/Km = 5.1 mM(-1) s(-1). The transcription levels of genes involved in nitrogen metabolism were up-regulated in the Δgad strain. The mutant showed approximately 4- and 8-fold increases in the transcript levels of kgd and gabdh encoding a novel α-ketoglutarate decarboxylase and γ-aminobutanal dehydrogenase, respectively. Overall results suggested that in Synechocystis lacking a functional GAD, the γ-aminobutanal dehydrogenase might serve as an alternative catalytic pathway for GABA synthesis
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a GABA metabolism
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|a Glutamate decarboxylase
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|a Synechocystis
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|a Transcription
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|a Δgad strain
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|a Glutamate Decarboxylase
|2 NLM
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|a EC 4.1.1.15
|2 NLM
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|a Nitrogen
|2 NLM
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| 650 |
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|a N762921K75
|2 NLM
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| 700 |
1 |
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|a Incharoensakdi, Aran
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 99(2016) vom: 03. Feb., Seite 59-65
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
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|g volume:99
|g year:2016
|g day:03
|g month:02
|g pages:59-65
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|u http://dx.doi.org/10.1016/j.plaphy.2015.12.008
|3 Volltext
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