Nonfouling NTA-PEG-Based TEM Grid Coatings for Selective Capture of Histidine-Tagged Protein Targets from Cell Lysates

We report the preparation and performance of TEM grids bearing stabilized nonfouling lipid monolayer coatings. These films contain NTA capture ligands of controllable areal density at the distal end of a flexible poly(ethylene glycol) 2000 (PEG2000) spacer to avoid preferred orientation of surface-b...

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Veröffentlicht in:	Langmuir : the ACS journal of surfaces and colloids. - 1992. - 32(2016), 2 vom: 19. Jan., Seite 551-9
1. Verfasser:	Benjamin, Christopher J (VerfasserIn)
Weitere Verfasser:	Wright, Kyle J, Hyun, Seok-Hee, Krynski, Kyle, Yu, Guimei, Bajaj, Ruchika, Guo, Fei, Stauffacher, Cynthia V, Jiang, Wen, Thompson, David H
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2016
Zugriff auf das übergeordnete Werk:	Langmuir : the ACS journal of surfaces and colloids
Schlagworte:	Journal Article Research Support, N.I.H., Extramural 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000) ATP-Binding Cassette Transporters Cell Extracts Escherichia coli Proteins His-His-His-His-His-His MPEG350 Oligopeptides Phosphatidylethanolamines mehr... Recombinant Fusion Proteins Ribosomal Proteins maltose transport system, E coli Green Fluorescent Proteins 147336-22-9 Polyethylene Glycols 3WJQ0SDW1A Histidine 4QD397987E ribosomal protein L7-L12 70815-33-7 polyethylene glycol 2000 HAF0412YIT Nitrilotriacetic Acid KA90006V9D