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231224s2016 xx |||||o 00| ||eng c |
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7 |
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|a 10.1021/acs.langmuir.5b03777
|2 doi
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|a pubmed24n0853.xml
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Lam, Michael K
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Tuning Toehold Length and Temperature to Achieve Rapid, Colorimetric Detection of DNA from the Disassembly of DNA-Gold Nanoparticle Aggregates
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 08.03.2017
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|a Date Revised 08.03.2017
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Gold nanoparticles have been widely utilized to achieve colorimetric detection for various diagnostic applications. One of the most frequently used methods for DNA detection involves the aggregation of DNA-modified gold nanoparticles driven by target DNA hybridization. This process, however, is intrinsically slow, limiting its use in rapid diagnostics. Here we take advantage of the reverse process: the disassembly of preformed aggregates triggered by the addition of target DNA via a strand displacement mechanism. A systematic study of the dependence of the disassembly rate on temperature, with and without toeholds, has delivered a system that produces an extremely rapid colorimetric response. Furthermore, using an optimal toehold length of 5 nucleotides, target triggered disassembly is rapid over a wide range of ambient temperatures. Using this overhang system, simple visualization of low picomole amounts of target DNA is possible within 10 min at room temperature
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Gold
|2 NLM
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|a 7440-57-5
|2 NLM
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|a DNA
|2 NLM
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| 650 |
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|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Gadzikwa, Tendai
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nguyen, Trang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kausar, Abu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Alladin-Mustan, B Safeenaz
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sikder, Md Delwar
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gibbs-Davis, Julianne M
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 32(2016), 6 vom: 16. Feb., Seite 1585-90
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:32
|g year:2016
|g number:6
|g day:16
|g month:02
|g pages:1585-90
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|u http://dx.doi.org/10.1021/acs.langmuir.5b03777
|3 Volltext
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