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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.5b02727
|2 doi
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|a pubmed24n0852.xml
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|a (DE-627)NLM255821395
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|a (NLM)26695478
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wiarachai, Oraphan
|e verfasserin
|4 aut
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|a Clickable and Antifouling Platform of Poly[(propargyl methacrylate)-ran-(2-methacryloyloxyethyl phosphorylcholine)] for Biosensing Applications
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 01.02.2017
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|a Date Revised 01.02.2017
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a A functional copolymer platform, namely, poly[(propargyl methacrylate)-ran-(2-methacryloyloxyethyl phosphorylcholine)] (PPgMAMPC), was synthesized by reversible addition-fragmentation chain-transfer polymerization. In principle, the alkyne moiety of propargyl methacrylate (PgMA) should serve as an active site for binding azide-containing molecules via a click reaction, i.e., Cu-catalyzed azide/alkyne cycloaddition (CuAAC), and 2-methacryloyloxyethyl phosphorylcholine (MPC), the hydrophilic monomeric unit, should enable the copolymer to suppress nonspecific adsorption. The copolymers were characterized using Fourier transform infrared (FTIR) and (1)H NMR spectroscopies. Thiol-terminated, PPgMAMPC-SH, obtained by aminolysis of PPgMAMPC, was immobilized on a gold-coated substrate using a "grafting to" approach via self-assembly. Azide-containing species, namely, biotin and peptide nucleic acid (PNA), were then immobilized on the alkyne-containing copolymeric platform via CuAAC. The potential use of surface-attached PPgMAMPC in biosensing applications was shown by detection of specific target molecules, i.e., streptavidin (SA) and DNA, by the developed sensing platform using a surface plasmon resonance technique. The copolymer composition strongly influenced the performance of the developed sensing platform in terms of signal-to-noise ratio in the case of the biotin-SA system and hybridization efficiency and mismatch discrimination for the PNA-DNA system
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Azides
|2 NLM
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|a DNA, Complementary
|2 NLM
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|a Methacrylates
|2 NLM
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|a Peptide Nucleic Acids
|2 NLM
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|a poly((propargyl methacrylate)-ran-(2-methacryloyloxyethyl phosphorylcholine))
|2 NLM
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|a Phosphorylcholine
|2 NLM
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|a 107-73-3
|2 NLM
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|a Biotin
|2 NLM
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|a 6SO6U10H04
|2 NLM
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|a Gold
|2 NLM
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| 650 |
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|a 7440-57-5
|2 NLM
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| 650 |
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|a Streptavidin
|2 NLM
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| 650 |
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|a 9013-20-1
|2 NLM
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| 700 |
1 |
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|a Vilaivan, Tirayut
|e verfasserin
|4 aut
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1 |
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|a Iwasaki, Yasuhiko
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hoven, Voravee P
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 32(2016), 4 vom: 02. Feb., Seite 1184-94
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:32
|g year:2016
|g number:4
|g day:02
|g month:02
|g pages:1184-94
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|u http://dx.doi.org/10.1021/acs.langmuir.5b02727
|3 Volltext
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