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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2015.11.010
|2 doi
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|a pubmed24n0852.xml
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|a (DE-627)NLM255779704
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|a (NLM)26691061
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|a (PII)S0981-9428(15)30165-0
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Santana, Juliano Oliveira
|e verfasserin
|4 aut
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| 245 |
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|a Characterization of the legumains encoded by the genome of Theobroma cacao L
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 27.09.2016
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2015 Universidade Estadual de Santa Cruz, CNPJ: 40738999/0001-95. Published by Elsevier Masson SAS.. All rights reserved.
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|a Legumains are cysteine proteases related to plant development, protein degradation, programmed cell death, and defense against pathogens. In this study, we have identified and characterized three legumains encoded by Theobroma cacao genome through in silico analyses, three-dimensional modeling, genetic expression pattern in different tissues and as a response to the inoculation of Moniliophthora perniciosa fungus. The three proteins were named TcLEG3, TcLEG6, and TcLEG9. Histidine and cysteine residue which are part of the catalytic site were conserved among the proteins, and they remained parallel in the loop region in the 3D modeling. Three-dimensional modeling showed that the propeptide, which is located in the terminal C region of legumains blocks the catalytic cleft. Comparing dendrogram data with the relative expression analysis, indicated that TcLEG3 is related to the seed legumain group, TcLEG6 is related with the group of embryogenesis activities, and protein TcLEG9, with processes regarding the vegetative group. Furthermore, the expression analyses proposes a significant role for the three legumains during the development of Theobroma cacao and in its interaction with M. perniciosa
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cocoa
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| 650 |
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|a Cysteine-proteinase
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|a Gene expression
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|a Modeling
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|a Witches' broom
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|a qPCR
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Cysteine Endopeptidases
|2 NLM
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| 650 |
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|a EC 3.4.22.-
|2 NLM
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| 650 |
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|a asparaginylendopeptidase
|2 NLM
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| 650 |
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|a EC 3.4.22.34
|2 NLM
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| 700 |
1 |
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|a Freire, Laís
|e verfasserin
|4 aut
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| 700 |
1 |
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|a de Sousa, Aurizangela Oliveira
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fontes Soares, Virgínia Lúcia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gramacho, Karina Peres
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pirovani, Carlos Priminho
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 98(2016) vom: 26. Jan., Seite 162-70
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:98
|g year:2016
|g day:26
|g month:01
|g pages:162-70
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|u http://dx.doi.org/10.1016/j.plaphy.2015.11.010
|3 Volltext
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|d 98
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