CYP74B24 is the 13-hydroperoxide lyase involved in biosynthesis of green leaf volatiles in tea (Camellia sinensis)

CYP74B24 is the 13-hydroperoxide lyase involved in biosynthesis of green leaf volatiles in tea (Camellia sinensis)

Copyright © 2015 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 98(2016) vom: 21. Jan., Seite 112-8
1. Verfasser: Ono, Eiichiro (VerfasserIn)
Weitere Verfasser: Handa, Taiki, Koeduka, Takao, Toyonaga, Hiromi, Tawfik, Moataz M, Shiraishi, Akira, Murata, Jun, Matsui, Kenji
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't CYP74 Camellia sinensis Green leaf volatile Hydroperoxide lyase Tea Acetates Aldehydes Linolenic Acids mehr... Lipid Peroxides Plant Proteins Recombinant Proteins Volatile Organic Compounds 13-hydroperoxy-9,11,15-octadecatrienoic acid 28836-09-1 3-hexenal 3300F8DZ5A Cytochrome P-450 Enzyme System 9035-51-2 Aldehyde-Lyases EC 4.1.2.- hydroperoxide lyase
Beschreibung
Zusammenfassung:Copyright © 2015 Elsevier Masson SAS. All rights reserved.
Green leaf volatiles (GLVs) are C6-aliphatic aldehydes/alcohols/acetates, and biosynthesized from the central precursor fatty acid 13-hydroperoxides by 13-hydroperoxide lyases (HPLs) in various plant species. While GLVs have been implicated as defense compounds in plants, GLVs give characteristic grassy note to a bouquet of aroma in green tea, which is manufactured from young leaves of Camellia sinensis. Here we identify three HPL-related genes from C. sinensis via RNA-Sequencing (RNA-Seq) in silico, and functionally characterized a candidate gene, CYP74B24, as a gene encoding tea HPL. Recombinant CYP74B24 protein heterologously expressed in Escherichia coli specifically produced (Z)-3-hexenal from 13-HPOT with the optimal pH 6.0 in vitro. CYP74B24 gene was expressed throughout the aerial organs in a rather constitutive manner and further induced by mechanical wounding. Constitutive expression of CYP74B24 gene in intact tea leaves might account for low but substantial and constitutive formation of a subset of GLVs, some of which are stored as glycosides. Our results not only provide novel insights into the biological roles that GLVs play in tea plants, but also serve as basis for the improvement of aroma quality in tea manufacturing processes
Beschreibung:Date Completed 27.09.2016
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2015.11.016