Non-targeted profiling of semi-polar metabolites in Arabidopsis root exudates uncovers a role for coumarin secretion and lignification during the local response to phosphate limitation

Non-targeted profiling of semi-polar metabolites in Arabidopsis root exudates uncovers a role for coumarin secretion and lignification during the local response to phosphate limitation

© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 67(2016), 5 vom: 15. März, Seite 1421-32
1. Verfasser: Ziegler, Jörg (VerfasserIn)
Weitere Verfasser: Schmidt, Stephan, Chutia, Ranju, Müller, Jens, Böttcher, Christoph, Strehmel, Nadine, Scheel, Dierk, Abel, Steffen
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Arabidopsis thaliana coumarin hydroponic system lignification metabolite profiling oligolignol phosphate starvation root exudates. mehr... Coumarins Malates Phosphates Plant Exudates Citric Acid 2968PHW8QP malic acid 817L1N4CKP Lignin 9005-53-2 Arginine 94ZLA3W45F A4VZ22K1WT Phloroglucinol DHD7FFG6YS
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100 1 |a Ziegler, Jörg  |e verfasserin  |4 aut 
245 1 0 |a Non-targeted profiling of semi-polar metabolites in Arabidopsis root exudates uncovers a role for coumarin secretion and lignification during the local response to phosphate limitation 
264 1 |c 2016 
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500 |a Date Completed 13.12.2016 
500 |a Date Revised 18.03.2024 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. 
520 |a Plants have evolved two major strategies to cope with phosphate (Pi) limitation. The systemic response, mainly comprising increased Pi uptake and metabolic adjustments for more efficient Pi use, and the local response, enabling plants to explore Pi-rich soil patches by reorganization of the root system architecture. Unlike previous reports, this study focused on root exudation controlled by the local response to Pi deficiency. To approach this, a hydroponic system separating the local and systemic responses was developed. Arabidopsis thaliana genotypes exhibiting distinct sensitivities to Pi deficiency could be clearly distinguished by their root exudate composition as determined by non-targeted reversed-phase ultraperformance liquid chromatography electrospray ionization quadrupole-time-of-flight mass spectrometry metabolite profiling. Compared with wild-type plants or insensitive low phosphate root 1 and 2 (lpr1 lpr2) double mutant plants, the hypersensitive phosphate deficiency response 2 (pdr2) mutant exhibited a reduced number of differential features in root exudates after Pi starvation, suggesting the involvement of PDR2-encoded P5-type ATPase in root exudation. Identification and analysis of coumarins revealed common and antagonistic regulatory pathways between Pi and Fe deficiency-induced coumarin secretion. The accumulation of oligolignols in root exudates after Pi deficiency was inversely correlated with Pi starvation-induced lignification at the root tips. The strongest oligolignol accumulation in root exudates was observed for the insensitive lpr1 lpr2 double mutant, which was accompanied by the absence of Pi deficiency-induced lignin deposition, suggesting a role of LPR ferroxidases in lignin polymerization during Pi starvation 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Arabidopsis thaliana 
650 4 |a coumarin 
650 4 |a hydroponic system 
650 4 |a lignification 
650 4 |a metabolite profiling 
650 4 |a oligolignol 
650 4 |a phosphate starvation 
650 4 |a root exudates. 
650 7 |a Coumarins  |2 NLM 
650 7 |a Malates  |2 NLM 
650 7 |a Phosphates  |2 NLM 
650 7 |a Plant Exudates  |2 NLM 
650 7 |a Citric Acid  |2 NLM 
650 7 |a 2968PHW8QP  |2 NLM 
650 7 |a malic acid  |2 NLM 
650 7 |a 817L1N4CKP  |2 NLM 
650 7 |a Lignin  |2 NLM 
650 7 |a 9005-53-2  |2 NLM 
650 7 |a Arginine  |2 NLM 
650 7 |a 94ZLA3W45F  |2 NLM 
650 7 |a coumarin  |2 NLM 
650 7 |a A4VZ22K1WT  |2 NLM 
650 7 |a Phloroglucinol  |2 NLM 
650 7 |a DHD7FFG6YS  |2 NLM 
700 1 |a Schmidt, Stephan  |e verfasserin  |4 aut 
700 1 |a Chutia, Ranju  |e verfasserin  |4 aut 
700 1 |a Müller, Jens  |e verfasserin  |4 aut 
700 1 |a Böttcher, Christoph  |e verfasserin  |4 aut 
700 1 |a Strehmel, Nadine  |e verfasserin  |4 aut 
700 1 |a Scheel, Dierk  |e verfasserin  |4 aut 
700 1 |a Abel, Steffen  |e verfasserin  |4 aut 
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