Comparative analysis of the tubulin cytoskeleton organization in nodules of Medicago truncatula and Pisum sativum : bacterial release and bacteroid positioning correlate with characteristic microtubule rearrangements

© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 210(2016), 1 vom: 16. Apr., Seite 168-83
1. Verfasser: Kitaeva, Anna B (VerfasserIn)
Weitere Verfasser: Demchenko, Kirill N, Tikhonovich, Igor A, Timmers, Antonius C J, Tsyganov, Viktor E
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't Medicago truncatula (barrel medic) Pisum sativum (pea) cell differentiation microtubules (MTs) nodulation symbiosis tubulin cytoskeleton Tubulin
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245 1 0 |a Comparative analysis of the tubulin cytoskeleton organization in nodules of Medicago truncatula and Pisum sativum  |b bacterial release and bacteroid positioning correlate with characteristic microtubule rearrangements 
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520 |a © 2015 The Authors. New Phytologist © 2015 New Phytologist Trust. 
520 |a In this study we analyzed and compared the organization of the tubulin cytoskeleton in nodules of Medicago truncatula and Pisum sativum. We combined antibody labeling and green fluorescent protein tagging with laser confocal microscopy to observe microtubules (MTs) in nodules of both wild-type (WT) plants and symbiotic plant mutants blocked at different steps of nodule development. The 3D MT organization of each histological nodule zone in both M. truncatula and P. sativum is correlated to specific developmental processes. Endoplasmic MTs appear to support infection thread growth, infection droplet formation and bacterial release into the host cytoplasm in nodules of both species. No differences in the organization of the MT cytoskeleton between WT and bacterial release mutants were apparent, suggesting both that the phenotype is not linked to a defect in MT organization and that the growth of hypertrophied infection threads is supported by MTs. Strikingly, bacterial release coincides with a change in the organization of cortical MTs from parallel arrays into an irregular, crisscross arrangement. After release, the organization of endoplasmic MTs is linked to the distribution of symbiosomes. The 3D MT organization of each nodule histological zone in M. truncatula and P. sativum was analyzed and linked to specific developmental processes 
650 4 |a Comparative Study 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Medicago truncatula (barrel medic) 
650 4 |a Pisum sativum (pea) 
650 4 |a cell differentiation 
650 4 |a microtubules (MTs) 
650 4 |a nodulation 
650 4 |a symbiosis 
650 4 |a tubulin cytoskeleton 
650 7 |a Tubulin  |2 NLM 
700 1 |a Demchenko, Kirill N  |e verfasserin  |4 aut 
700 1 |a Tikhonovich, Igor A  |e verfasserin  |4 aut 
700 1 |a Timmers, Antonius C J  |e verfasserin  |4 aut 
700 1 |a Tsyganov, Viktor E  |e verfasserin  |4 aut 
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773 1 8 |g volume:210  |g year:2016  |g number:1  |g day:16  |g month:04  |g pages:168-83 
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