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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.5b02862
|2 doi
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|a pubmed24n0852.xml
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|a (DE-627)NLM25569055X
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|a (NLM)26681447
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Koeda, Shuhei
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Construction and Characterization of Protein-Encapsulated Electrospun Fibermats Prepared from a Silica/Poly(γ-glutamate) Hybrid
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 17.10.2016
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|a Date Revised 16.11.2017
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Protein-encapsulated fibermats are an attractive platform for protein-based bioactive materials. However, the choice of methods is still limited and not applicable to a wide range of proteins. In this study, we studied new polymeric materials for constructing protein-encapsulated fibermats, in which protein molecules are encapsulated within the nanofibers of fibermats without causing deleterious changes to protein structure or function. We constructed a protein-encapsulated fibermat using the poly(γ-glutamate) (PGA)/(3-glycidyloxypropyl)-trimethoxysilane (GPTMS) hybrid as a precursor for electrospinning. Because the PGA/GPTMS hybrid is water-soluble, protein molecules can be added to the precursor in an aqueous solution, significantly enhancing protein stability. Polycondensation during electrospinning (in-flight polycondensation) makes the obtained fibermats water-insoluble, which stabilizes the fibermat structure such that it is resistant to degradation in aqueous buffer. The molecular structure of the PGA/GPTMS hybrid gives rise to unique molecular permeability, which alters the selectivity and specificity of biochemical reactions involving the encapsulated enzymes; lower molecular-weight (MW) substrates can permeate the nanofibers, promoting enzyme activity, but higher MW substrates such as inhibitor peptides cannot permeate the nanofibers, suppressing enzyme activity. We present an effective method of encapsulating bioactive molecules while maintaining their structure and function, increasing the versatility of electrospun fibermats for constructing various bioactive materials
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Proteins
|2 NLM
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|a Silanes
|2 NLM
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|a poly(gamma-glutamic acid)
|2 NLM
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|a Polyglutamic Acid
|2 NLM
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| 650 |
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|a 25513-46-6
|2 NLM
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| 650 |
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|a Silicon Dioxide
|2 NLM
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| 650 |
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|a 7631-86-9
|2 NLM
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| 650 |
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|a trimethoxysilane
|2 NLM
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| 650 |
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7 |
|a V1J39XPF91
|2 NLM
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| 700 |
1 |
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|a Ichiki, Kentaro
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Iwanaga, Norihiko
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mizuno, Koji
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shibata, Masahide
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Obata, Akiko
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kasuga, Toshihiro
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mizuno, Toshihisa
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 32(2016), 1 vom: 12. Jan., Seite 221-9
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:32
|g year:2016
|g number:1
|g day:12
|g month:01
|g pages:221-9
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| 856 |
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|u http://dx.doi.org/10.1021/acs.langmuir.5b02862
|3 Volltext
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