Arabinogalactan proteins have deep roots in eukaryotes identification of genes and epitopes in brown algae and their role in Fucus serratus embryo development

Arabinogalactan proteins have deep roots in eukaryotes : identification of genes and epitopes in brown algae and their role in Fucus serratus embryo development

© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 209(2016), 4 vom: 30. März, Seite 1428-41
1. Verfasser: Hervé, Cécile (VerfasserIn)
Weitere Verfasser: Siméon, Amandine, Jam, Murielle, Cassin, Andrew, Johnson, Kim L, Salmeán, Armando A, Willats, William G T, Doblin, Monika S, Bacic, Antony, Kloareg, Bernard
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Ectocarpus Fucus embryo arabinogalactan proteins (AGPs) brown algae cell elongation cell polarity cell wall cell wall evolution mehr... Epitopes Indicators and Reagents Mucoproteins Plant Proteins arabinogalactan proteins
Beschreibung
Zusammenfassung:© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.
Arabinogalactan proteins (AGPs) are highly glycosylated, hydroxyproline-rich proteins found at the cell surface of plants, where they play key roles in developmental processes. Brown algae are marine, multicellular, photosynthetic eukaryotes. They belong to the phylum Stramenopiles, which is unrelated to land plants and green algae (Chloroplastida). Brown algae share common evolutionary features with other multicellular organisms, including a carbohydrate-rich cell wall. They differ markedly from plants in their cell wall composition, and AGPs have not been reported in brown algae. Here we investigated the presence of chimeric AGP-like core proteins in this lineage. We report that the genome sequence of the brown algal model Ectocarpus siliculosus encodes AGP protein backbone motifs, in a gene context that differs considerably from what is known in land plants. We showed the occurrence of AGP glycan epitopes in a range of brown algal cell wall extracts. We demonstrated that these chimeric AGP-like core proteins are developmentally regulated in embryos of the order Fucales and showed that AGP loss of function seriously impairs the course of early embryogenesis. Our findings shine a new light on the role of AGPs in cell wall sensing and raise questions about the origin and evolution of AGPs in eukaryotes
Beschreibung:Date Completed 19.12.2016
Date Revised 09.01.2024
published: Print-Electronic
CommentIn: New Phytol. 2016 Mar;209(4):1341-3. - PMID 26840249
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.13786