FLOURY ENDOSPERM7 encodes a regulator of starch synthesis and amyloplast development essential for peripheral endosperm development in rice

FLOURY ENDOSPERM7 encodes a regulator of starch synthesis and amyloplast development essential for peripheral endosperm development in rice

© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 67(2016), 3 vom: 25. Feb., Seite 633-47
1. Verfasser: Zhang, Long (VerfasserIn)
Weitere Verfasser: Ren, Yulong, Lu, Bingyue, Yang, Chunyan, Feng, Zhiming, Liu, Zhou, Chen, Jun, Ma, Weiwei, Wang, Ying, Yu, Xiaowen, Wang, Yunlong, Zhang, Wenwei, Wang, Yihua, Liu, Shijia, Wu, Fuqing, Zhang, Xin, Guo, Xiuping, Bao, Yiqun, Jiang, Ling, Wan, Jianmin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Amyloplast DUF1338 FLOURY ENDOSPERM7 endosperm cells rice (Oryza sativa) starch synthesis. Plant Proteins Green Fluorescent Proteins mehr... 147336-22-9 Starch 9005-25-8
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245 1 0 |a FLOURY ENDOSPERM7 encodes a regulator of starch synthesis and amyloplast development essential for peripheral endosperm development in rice 
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500 |a Date Revised 26.04.2022 
500 |a published: Print-Electronic 
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520 |a © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. 
520 |a In cereal crops, starch synthesis and storage depend mainly on a specialized class of plastids, termed amyloplasts. Despite the importance of starch, the molecular machinery regulating starch synthesis and amyloplast development remains largely unknown. Here, we report the characterization of the rice (Oryza sativa) floury endosperm7 (flo7) mutant, which develops a floury-white endosperm only in the periphery and not in the inner portion. Consistent with the phenotypic alternation in flo7 endosperm, the flo7 mutant had reduced amylose content and seriously disrupted amylopectin structure only in the peripheral endosperm. Notably, flo7 peripheral endosperm cells showed obvious defects in compound starch grain development. Map-based cloning of FLO7 revealed that it encodes a protein of unknown function. FLO7 harbors an N-terminal transit peptide capable of targeting functional FLO7 fused to green fluorescent protein to amyloplast stroma in developing endosperm cells, and a domain of unknown function 1338 (DUF1338) that is highly conserved in green plants. Furthermore, our combined β-glucuronidase activity and RNA in situ hybridization assays showed that the FLO7 gene was expressed ubiquitously but exhibited a specific expression in the endosperm periphery. Moreover, a set of in vivo experiments demonstrated that the missing 32 aa in the flo7 mutant protein are essential for the stable accumulation of FLO7 in the endosperm. Together, our findings identify FLO7 as a unique plant regulator required for starch synthesis and amyloplast development within the peripheral endosperm and provide new insights into the spatial regulation of endosperm development in rice 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Amyloplast 
650 4 |a DUF1338 
650 4 |a FLOURY ENDOSPERM7 
650 4 |a endosperm cells 
650 4 |a rice (Oryza sativa) 
650 4 |a starch synthesis. 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Green Fluorescent Proteins  |2 NLM 
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650 7 |a 9005-25-8  |2 NLM 
700 1 |a Ren, Yulong  |e verfasserin  |4 aut 
700 1 |a Lu, Bingyue  |e verfasserin  |4 aut 
700 1 |a Yang, Chunyan  |e verfasserin  |4 aut 
700 1 |a Feng, Zhiming  |e verfasserin  |4 aut 
700 1 |a Liu, Zhou  |e verfasserin  |4 aut 
700 1 |a Chen, Jun  |e verfasserin  |4 aut 
700 1 |a Ma, Weiwei  |e verfasserin  |4 aut 
700 1 |a Wang, Ying  |e verfasserin  |4 aut 
700 1 |a Yu, Xiaowen  |e verfasserin  |4 aut 
700 1 |a Wang, Yunlong  |e verfasserin  |4 aut 
700 1 |a Zhang, Wenwei  |e verfasserin  |4 aut 
700 1 |a Wang, Yihua  |e verfasserin  |4 aut 
700 1 |a Liu, Shijia  |e verfasserin  |4 aut 
700 1 |a Wu, Fuqing  |e verfasserin  |4 aut 
700 1 |a Zhang, Xin  |e verfasserin  |4 aut 
700 1 |a Guo, Xiuping  |e verfasserin  |4 aut 
700 1 |a Bao, Yiqun  |e verfasserin  |4 aut 
700 1 |a Jiang, Ling  |e verfasserin  |4 aut 
700 1 |a Wan, Jianmin  |e verfasserin  |4 aut 
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