Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting

Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting

© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Bibliographische Detailangaben
Veröffentlicht in:Advanced materials (Deerfield Beach, Fla.). - 1998. - 28(2016), 2 vom: 13. Jan., Seite 327-334
1. Verfasser: Duncombe, Todd A (VerfasserIn)
Weitere Verfasser: Kang, Chi-Chih, Maity, Santanu, Ward, Toby M, Pegram, Mark D, Murthy, Niren, Herr, Amy E
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Advanced materials (Deerfield Beach, Fla.)
Schlagworte:Evaluation Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. cancer therapies gradient gels grayscale masks hydrogel pore expansion western blots mehr... EIF4ENIF1 protein, human Hydrogels Nucleocytoplasmic Transport Proteins Green Fluorescent Proteins 147336-22-9 MTOR protein, human EC 2.7.1.1 ERBB2 protein, human EC 2.7.10.1 Receptor, ErbB-2 TOR Serine-Threonine Kinases EC 2.7.11.1 Extracellular Signal-Regulated MAP Kinases EC 2.7.11.24
Beschreibung
Zusammenfassung:© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Pore-gradient microgel arrays enable thousands of parallel high-resolution single-cell protein electrophoresis separations for targets accross a wide molecular mass (25-289 kDa), yet within 1 mm separation distances. Dual crosslinked hydrogels facilitate gel-pore expansion after electrophoresis for efficient and uniform immunoprobing. The photopatterned, light-activated, and acid-expandable hydrogel underpins single-cell protein analysis, here for oncoprotein-related signaling in human breast biopsy
Beschreibung:Date Completed 15.12.2016
Date Revised 03.12.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-4095
DOI:10.1002/adma.201503939