Genomic-assisted phylogenetic analysis and marker development for next generation soybean cyst nematode resistance breeding

Copyright © 2015 The Authors. Published by Elsevier Ireland Ltd.. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant science : an international journal of experimental plant biology. - 1985. - 242(2016) vom: 01. Jan., Seite 342-350
1. Verfasser: Kadam, Suhas (VerfasserIn)
Weitere Verfasser: Vuong, Tri D, Qiu, Dan, Meinhardt, Clinton G, Song, Li, Deshmukh, Rupesh, Patil, Gunvant, Wan, Jinrong, Valliyodan, Babu, Scaboo, Andrew M, Shannon, J Grover, Nguyen, Henry T
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Plant science : an international journal of experimental plant biology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Copy Number Variation Digital-PCR assay KASPar assay Phylogenetic diversity SCN resistance genes/QTL SNP genotyping Soybean Genetic Markers
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520 |a Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is a serious soybean pest. The use of resistant cultivars is an effective approach for preventing yield loss. In this study, 19,652 publicly available soybean accessions that were previously genotyped with the SoySNP50K iSelect BeadChip were used to evaluate the phylogenetic diversity of SCN resistance genes Rhg1 and Rhg4 in an attempt to identify novel sources of resistance. The sequence information of soybean lines was utilized to develop KASPar (KBioscience Competitive Allele-Specific PCR) assays from single nucleotide polymorphisms (SNPs) of Rhg1, Rhg4, and other novel quantitative trait loci (QTL). These markers were used to genotype a diverse set of 95 soybean germplasm lines and three recombinant inbred line (RIL) populations. SNP markers from the Rhg1 gene were able to differentiate copy number variation (CNV), such as resistant-high copy (PI 88788-type), low copy (Peking-type), and susceptible-single copy (Williams 82) numbers. Similarly, markers for the Rhg4 gene were able to detect Peking-type (resistance) genotypes. The phylogenetic information of SCN resistance loci from a large set of soybean accessions and the gene/QTL specific markers that were developed in this study will accelerate SCN resistance breeding programs 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Copy Number Variation 
650 4 |a Digital-PCR assay 
650 4 |a KASPar assay 
650 4 |a Phylogenetic diversity 
650 4 |a SCN resistance genes/QTL 
650 4 |a SNP genotyping 
650 4 |a Soybean 
650 7 |a Genetic Markers  |2 NLM 
700 1 |a Vuong, Tri D  |e verfasserin  |4 aut 
700 1 |a Qiu, Dan  |e verfasserin  |4 aut 
700 1 |a Meinhardt, Clinton G  |e verfasserin  |4 aut 
700 1 |a Song, Li  |e verfasserin  |4 aut 
700 1 |a Deshmukh, Rupesh  |e verfasserin  |4 aut 
700 1 |a Patil, Gunvant  |e verfasserin  |4 aut 
700 1 |a Wan, Jinrong  |e verfasserin  |4 aut 
700 1 |a Valliyodan, Babu  |e verfasserin  |4 aut 
700 1 |a Scaboo, Andrew M  |e verfasserin  |4 aut 
700 1 |a Shannon, J Grover  |e verfasserin  |4 aut 
700 1 |a Nguyen, Henry T  |e verfasserin  |4 aut 
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