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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2015.10.034
|2 doi
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|a pubmed25n0848.xml
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|a (PII)S0981-9428(15)30154-6
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|e rakwb
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|a eng
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|a Feki, Kaouthar
|e verfasserin
|4 aut
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|a Multiple abiotic stress tolerance of the transformants yeast cells and the transgenic Arabidopsis plants expressing a novel durum wheat catalase
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 09.01.2017
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2015 Elsevier Masson SAS. All rights reserved.
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|a Catalases are reactive oxygen species scavenging enzymes involved in response to abiotic and biotic stresses. In this study, we described the isolation and functional characterization of a novel catalase from durum wheat, designed TdCAT1. Molecular Phylogeny analyses showed that wheat TdCAT1 exhibited high amino acids sequence identity to other plant catalases. Sequence homology analysis showed that TdCAT1 protein contained the putative calmodulin binding domain and a putative conserved internal peroxisomal targeting signal PTS1 motif around its C-terminus. Predicted three-dimensional structural model revealed the presence of four putative distinct structural regions which are the N-terminal arm, the β-barrel, the wrapping and the α-helical domains. TdCAT1 protein had the heme pocket that was composed by five essential residues. TdCAT1 gene expression analysis showed that this gene was induced by various abiotic stresses in durum wheat. The expression of TdCAT1 in yeast cells and Arabidopsis plants conferred tolerance to several abiotic stresses. Compared with the non-transformed plants, the transgenic lines maintained their growth and accumulated more proline under stress treatments. Furthermore, the amount of H2O2 was lower in transgenic lines, which was due to the high CAT and POD activities. Taken together, these data provide the evidence for the involvement of durum wheat catalase TdCAT1 in tolerance to multiple abiotic stresses in crop plants
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Abiotic stress
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|a Antioxidant enzymes
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|a Arabidopsis
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|a Catalase
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|a Oxidative stress
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|a Triticum durum
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|a Antioxidants
|2 NLM
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|a Plant Proteins
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|a RNA, Messenger
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|a Proline
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|a 9DLQ4CIU6V
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|a Hydrogen Peroxide
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|a Catalase
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|a EC 1.11.1.6
|2 NLM
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|a Kamoun, Yosra
|e verfasserin
|4 aut
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|a Ben Mahmoud, Rihem
|e verfasserin
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|a Farhat-Khemakhem, Ameny
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|a Gargouri, Ali
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|a Brini, Faiçal
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 97(2015) vom: 10. Dez., Seite 420-31
|w (DE-627)NLM098178261
|x 1873-2690
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|g volume:97
|g year:2015
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|g month:12
|g pages:420-31
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|u http://dx.doi.org/10.1016/j.plaphy.2015.10.034
|3 Volltext
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