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|a eng
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| 100 |
1 |
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|a Liu, C
|e verfasserin
|4 aut
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| 245 |
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|a Improving z-tracking accuracy in the two-photon single-particle tracking microscope
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|c 2015
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Revised 30.09.2020
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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| 520 |
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|a Here, we present a method that can improve the z-tracking accuracy of the recently invented TSUNAMI (Tracking of Single particles Using Nonlinear And Multiplexed Illumination) microscope. This method utilizes a maximum likelihood estimator (MLE) to determine the particle's 3D position that maximizes the likelihood of the observed time-correlated photon count distribution. Our Monte Carlo simulations show that the MLE-based tracking scheme can improve the z-tracking accuracy of TSUNAMI microscope by 1.7 fold. In addition, MLE is also found to reduce the temporal correlation of the z-tracking error. Taking advantage of the smaller and less temporally correlated z-tracking error, we have precisely recovered the hybridization-melting kinetics of a DNA model system from thousands of short single-particle trajectories in silico. Our method can be generally applied to other 3D single-particle tracking techniques
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| 650 |
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|a Journal Article
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| 700 |
1 |
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|a Liu, Y-L
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Perillo, E P
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jiang, N
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dunn, A K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yeh, H-C
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Applied physics letters
|d 1998
|g 107(2015), 15 vom: 12. Okt., Seite 153701
|w (DE-627)NLM098165984
|x 0003-6951
|7 nnas
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| 773 |
1 |
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|g volume:107
|g year:2015
|g number:15
|g day:12
|g month:10
|g pages:153701
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|d 107
|j 2015
|e 15
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