|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM253926246 |
| 003 |
DE-627 |
| 005 |
20250219065320.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2015 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.plaphy.2015.10.014
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n0846.xml
|
| 035 |
|
|
|a (DE-627)NLM253926246
|
| 035 |
|
|
|a (NLM)26492131
|
| 035 |
|
|
|a (PII)S0981-9428(15)30134-0
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Zalutskaya, Zhanneta
|e verfasserin
|4 aut
|
| 245 |
1 |
4 |
|a The Chlamydomonas reinhardtii alternative oxidase 1 is regulated by heat stress
|
| 264 |
|
1 |
|c 2015
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 09.01.2017
|
| 500 |
|
|
|a Date Revised 30.09.2020
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2015 Elsevier Masson SAS. All rights reserved.
|
| 520 |
|
|
|a The alternative oxidase (AOX) is a non-energy conserving terminal oxidase that has emerged as an important mitochondrial component of the cell stress responses. Although the most studied abiotic condition in relation to Chlamydomonas reinhardtii is high temperature, changes in AOX capacity of the alga were studied only under oxidative stress and cold. To examine whether elevated temperatures affected AOX1 expression, we applied quantitative real-time PCR and pharmaceutical approaches. In this work, we demonstrated a sharp increase in AOX1 transcript and protein abundance under heat stress. Furthermore, C. reinhardtii cells displayed a large increase in alternative respiration in response to high temperature. Feeding with the protein kinase inhibitor staurosporine strongly retarded the AOX1 transcription. Finally, the addition of the calcium chelator EGTA prevented heat-induced AOX1 expression. Together, our results imply that heat-inducible Ca(2+) influx and protein kinase(s) may mediate AOX1 expression at elevated temperatures. Characterization of heat-induced AOX1 regulation in the green alga C. reinhardtii provides a framework for a more complete understanding of the function of this conserved protein
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Alternative oxidase
|
| 650 |
|
4 |
|a Calcium
|
| 650 |
|
4 |
|a Chlamydomonas reinhardtii
|
| 650 |
|
4 |
|a Heat stress
|
| 650 |
|
4 |
|a Inhibitor feeding
|
| 650 |
|
4 |
|a Quantitative real-time RT-PCR
|
| 650 |
|
7 |
|a DNA-Binding Proteins
|2 NLM
|
| 650 |
|
7 |
|a Heat Shock Transcription Factors
|2 NLM
|
| 650 |
|
7 |
|a Mitochondrial Proteins
|2 NLM
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a RNA, Messenger
|2 NLM
|
| 650 |
|
7 |
|a Transcription Factors
|2 NLM
|
| 650 |
|
7 |
|a Egtazic Acid
|2 NLM
|
| 650 |
|
7 |
|a 526U7A2651
|2 NLM
|
| 650 |
|
7 |
|a Oxidoreductases
|2 NLM
|
| 650 |
|
7 |
|a EC 1.-
|2 NLM
|
| 650 |
|
7 |
|a alternative oxidase
|2 NLM
|
| 650 |
|
7 |
|a EC 1.-
|2 NLM
|
| 650 |
|
7 |
|a Staurosporine
|2 NLM
|
| 650 |
|
7 |
|a H88EPA0A3N
|2 NLM
|
| 650 |
|
7 |
|a Calcium
|2 NLM
|
| 650 |
|
7 |
|a SY7Q814VUP
|2 NLM
|
| 700 |
1 |
|
|a Lapina, Tatiana
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Ermilova, Elena
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 97(2015) vom: 11. Dez., Seite 229-34
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
|
| 773 |
1 |
8 |
|g volume:97
|g year:2015
|g day:11
|g month:12
|g pages:229-34
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.plaphy.2015.10.014
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 97
|j 2015
|b 11
|c 12
|h 229-34
|