Alanine aminotransferase 1 (OsAlaAT1) plays an essential role in the regulation of starch storage in rice endosperm
Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.
| Publié dans: | Plant science : an international journal of experimental plant biology. - 1985. - 240(2015) vom: 16. Nov., Seite 79-89 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2015
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| Accès à la collection: | Plant science : an international journal of experimental plant biology |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Alanine aminotransferase Carbon metabolism Floury endosperm Rice Starch synthesis Plant Proteins Amylose 9005-82-7 plus... |
| Résumé: | Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. Alteration of storage substances, in particular the major storage form starch, leads to floury endosperm. Because floury mutants have physical attributes for milling processes, identification and characterization of those mutants are valuable. In this study we identified a floury endosperm mutant caused by a T-DNA insertion in Oryza sativa alanine-aminotransferase1 (OsAlaAT1). OsAlaAT1 is localized in the cytosol and has aminotransferase enzyme activity. The osalaat1 mutant has less amylose and its amylopectin is structurally altered. OsAlaAT1 is predominantly expressed in developing seeds during active starch synthesis. AlaAT catalyzes the interconversion of pyruvate to alanine, and this pathway is activated under low-oxygen conditions. Consistently, OsAlaAT1 is induced by such conditions. Expression of the starch synthesis genes AGPases, OsSSI, OsSSIIa, and OsPPDKB is decreased in the mutant. Thus, our observations suggest that OsAlaAT1 plays an essential role in starch synthesis in developing seeds that are exposed to low concentrations of oxygen |
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| Description: | Date Completed 02.08.2016 Date Revised 10.03.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2259 |
| DOI: | 10.1016/j.plantsci.2015.07.027 |