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231224s2015 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2015.08.019
|2 doi
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|a pubmed24n0845.xml
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|a (DE-627)NLM253762456
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|a (NLM)26475183
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|a (PII)S0168-9452(15)30051-0
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Zalutskaya, Zhanneta
|e verfasserin
|4 aut
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| 245 |
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|a Reduction of PII signaling protein enhances lipid body production in Chlamydomonas reinhardtii
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|c 2015
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 02.08.2016
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|a Date Revised 10.04.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.
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|a In all examined organisms that have the PII signal transduction machinery, PII coordinates the central C/N anabolic metabolism. In green algae and land plants, PII is localized in the chloroplast and controls the L-arginine biosynthetic pathway pathway. To elucidate additional functions of PII in the model photosynthetic organism Chlamydomonas reinhardtii (CrPII), we generated and analyzed four strains, in which PII was strongly under-expressed by artificial microRNA (GLB1-amiRNA strains). In response to nitrogen deficiency, Chlamydomonas produces triacylglycerols (TAGs) that are accumulated in lipid bodies (LB). Quantification of LBs by confocal microscopy in four GLB1-amiRNA strains showed that reduced PII levels resulted in over-accumulation of LBs compared to their parental strains. Moreover, knock-down of PII caused also an increase in the total TAG level. We propose that the larger yields of TAG-filled LBs in N-starved GLB1-amiRNA cells can be attributed to the strain's depleted PII level and their inability to properly control acetyl-CoA carboxylase activity (ACCase). Together, our results imply that PII in Chlamydomonas negatively controls TAG accumulation in LBs during acclimation to nitrogen starvation of the alga
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Artificial microRNA approach
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|a Chlamydomonas reinhardtii
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|a Lipid bodies
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|a PII signal transduction protein
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|a Triacylglycerol biosynthesis
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|a Algal Proteins
|2 NLM
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|a PII Nitrogen Regulatory Proteins
|2 NLM
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|a Triglycerides
|2 NLM
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|a Nitrogen
|2 NLM
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|a N762921K75
|2 NLM
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| 700 |
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|a Kharatyan, Nina
|e verfasserin
|4 aut
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|a Forchhammer, Karl
|e verfasserin
|4 aut
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| 700 |
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|a Ermilova, Elena
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 240(2015) vom: 16. Nov., Seite 1-9
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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| 773 |
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|g volume:240
|g year:2015
|g day:16
|g month:11
|g pages:1-9
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|u http://dx.doi.org/10.1016/j.plantsci.2015.08.019
|3 Volltext
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|d 240
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